Literature DB >> 30460562

Agrobacterium-Mediated Transformation of Barley Immature Embryos.

Alison Hinchliffe1, Wendy A Harwood2.   

Abstract

Barley transformation is an essential tool for a range of functional genomics studies as well as for future crop improvement. The demand for efficient crop transformation systems continues to grow, with new genome editing technologies adding to that demand. Here we describe an efficient and routine transformation protocol for the spring barley Golden Promise, based on Agrobacterium-mediated inoculation of immature embryos. This protocol has been widely used for overexpression and RNAi applications and more recently for CRISPR/Cas9 mediated genome editing. Average transformation efficiencies of 25% can be easily achieved.

Entities:  

Keywords:  Agrobacterium tumefaciens; Barley transformation; Genome editing; Genome engineering; Immature embryos

Mesh：

Substances：

Year:  2019        PMID： 30460562     DOI： 10.1007/978-1-4939-8944-7_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

1.  In-planta Gene Targeting in Barley Using Cas9 With and Without Geminiviral Replicons.

Authors:  Tom Lawrenson; Alison Hinchliffe; Martha Clarke; Yvie Morgan; Wendy Harwood
Journal:  Front Genome Ed       Date:  2021-06-15

2.  The TCP transcription factor HvTB2 heterodimerizes with VRS5 and controls spike architecture in barley.

Authors:  Tatiana de Souza Moraes; Sam W van Es; Inmaculada Hernández-Pinzón; Gwendolyn K Kirschner; Froukje van der Wal; Sylvia Rodrigues da Silveira; Jacqueline Busscher-Lange; Gerco C Angenent; Matthew Moscou; Richard G H Immink; G Wilma van Esse
Journal:  Plant Reprod       Date:  2022-03-07       Impact factor: 4.217

Review 3.  New Insights Into Tissue Culture Plant-Regeneration Mechanisms.

Authors:  Yun Long; Yun Yang; Guangtang Pan; Yaou Shen
Journal:  Front Plant Sci       Date:  2022-06-30       Impact factor: 6.627

4.  Excess nitrogen responsive HvMADS27 transcription factor controls barley root architecture by regulating abscisic acid level.

Authors:  Aleksandra Smoczynska; Andrzej Pacak; Aleksandra Grabowska; Dawid Bielewicz; Marcin Zadworny; Kashmir Singh; Jakub Dolata; Mateusz Bajczyk; Przemyslaw Nuc; Jacek Kesy; Magdalena Wozniak; Izabela Ratajczak; Wendy Harwood; Wojciech M Karlowski; Artur Jarmolowski; Zofia Szweykowska-Kulinska
Journal:  Front Plant Sci       Date:  2022-09-12       Impact factor: 6.627

5.  TRA1: A Locus Responsible for Controlling Agrobacterium-Mediated Transformability in Barley.

Authors:  Beata Orman-Ligeza; Wendy Harwood; Pete E Hedley; Alison Hinchcliffe; Malcolm Macaulay; Cristobal Uauy; Kay Trafford
Journal:  Front Plant Sci       Date:  2020-04-16       Impact factor: 5.753

6.  An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.).

Authors:  Sadiye Hayta; Mark A Smedley; Selcen U Demir; Robert Blundell; Alison Hinchliffe; Nicola Atkinson; Wendy A Harwood
Journal:  Plant Methods       Date:  2019-10-26       Impact factor: 4.993

7.  The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley.

Authors:  M Asyraf Md Hatta; Sanu Arora; Sreya Ghosh; Oadi Matny; Mark A Smedley; Guotai Yu; Soma Chakraborty; Dhara Bhatt; Xiaodi Xia; Burkhard Steuernagel; Terese Richardson; Rohit Mago; Evans S Lagudah; Nicola J Patron; Michael Ayliffe; Matthew N Rouse; Wendy A Harwood; Sambasivam Periyannan; Brian J Steffenson; Brande B H Wulff
Journal:  Plant Biotechnol J       Date:  2020-09-06       Impact factor: 9.803

8.  The Effect of Endogenous Hormones, Total Antioxidant and Total Phenol Changes on Regeneration of Barley Cultivars.

Authors:  Ali Saeedpour; Sodabeh Jahanbakhsh Godehkahriz; Tahmineh Lohrasebi; Kasra Esfahani; Ali Hatef Salmanian; Khadijeh Razavi
Journal:  Iran J Biotechnol       Date:  2021-01-01       Impact factor: 1.671
  8 in total

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