M A Mohammed1, B E Aboulhoda2, R H Mahmoud3. 1. 1 Department of Physiology, Faculty of Medicine, Beni-Suef University, Beni Suef, Egypt. 2. 2 Department of Anatomy, Faculty of Medicine, Cairo University, Giza, Egypt. 3. 3 Department of Biochemistry, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Abstract
BACKGROUND: Despite being one of the most nephrotoxic drugs, gentamicin (GM) remains a mainstay as a first-choice agent in a vast variety of clinical situations owing to its superlative efficiency as a broad-spectrum antibiotic in treating several life-threatening bacterial infections. This urgently calls for the need for in-depth analysis of the mechanisms governing GM-induced nephrotoxicity and entails the necessity of presenting novel protective agents capable of ameliorating those renal deleterious effects. The reactive oxygen species and redox-sensitive transcription factors in GM-induced nephrotoxicity have recently called attention. PURPOSE: This study has been designed to shed light on the possible mechanisms of GM-induced nephrotoxicity and to provide a consensus set of histopathological, immunohistochemical, genetic and biochemical parameters elucidating the protective role of vitamin D against this nephrotoxicity. METHODS: Twenty-four adult male albino rats were equally divided into four groups: group I (control group), group II (GM), group III (GM + vitamin D) and group IV (vitamin D only). Kidney function tests, histopathological examination, gene expression of nuclear factor 2, nuclear factor kappa beta (NF-κB) and western blot of NF-κB p65, assessment of glutathione peroxidase and nicotinamide adenine dinucleotide phosphate oxidase (NADPH) oxidase by ELISA, as well as immunohistochemical evaluation of inducible nitric oxide, malondialdehyde, 8-hydroxy 2 deoxyguanine and vitamin D receptor, have been carried out. RESULTS: The kidney function deterioration, tissue oxidative stress development and the histopathological changes induced by GM were significantly attenuated by vitamin D administration. CONCLUSION: Vitamin D attenuates GM nephrotoxicity through its antioxidant properties and prevention of DNA damage.
BACKGROUND: Despite being one of the most nephrotoxic drugs, gentamicin (GM) remains a mainstay as a first-choice agent in a vast variety of clinical situations owing to its superlative efficiency as a broad-spectrum antibiotic in treating several life-threatening bacterial infections. This urgently calls for the need for in-depth analysis of the mechanisms governing GM-induced nephrotoxicity and entails the necessity of presenting novel protective agents capable of ameliorating those renal deleterious effects. The reactive oxygen species and redox-sensitive transcription factors in GM-induced nephrotoxicity have recently called attention. PURPOSE: This study has been designed to shed light on the possible mechanisms of GM-induced nephrotoxicity and to provide a consensus set of histopathological, immunohistochemical, genetic and biochemical parameters elucidating the protective role of vitamin D against this nephrotoxicity. METHODS: Twenty-four adult male albino rats were equally divided into four groups: group I (control group), group II (GM), group III (GM + vitamin D) and group IV (vitamin D only). Kidney function tests, histopathological examination, gene expression of nuclear factor 2, nuclear factor kappa beta (NF-κB) and western blot of NF-κB p65, assessment of glutathione peroxidase and nicotinamide adenine dinucleotide phosphate oxidase (NADPH) oxidase by ELISA, as well as immunohistochemical evaluation of inducible nitric oxide, malondialdehyde, 8-hydroxy 2 deoxyguanine and vitamin D receptor, have been carried out. RESULTS: The kidney function deterioration, tissue oxidative stress development and the histopathological changes induced by GM were significantly attenuated by vitamin D administration. CONCLUSION: Vitamin D attenuates GM nephrotoxicity through its antioxidant properties and prevention of DNA damage.
Entities:
Keywords:
Gentamicin; nephrotoxicity; oxidative stress; vitamin D
Authors: Eman S Said; Radwa M Ahmed; Rehab A Mohammed; Enas M Morsi; Mohamed H Elmahdi; Hassan S Elsayed; Rania H Mahmoud; Eman H Nadwa Journal: Heliyon Date: 2021-07-06
Authors: Ghada A Abdel-Latif; Azza H Abd Elwahab; Rehab A Hasan; Noura F ElMongy; Maggie M Ramzy; Manal L Louka; Mona F Schaalan Journal: Sci Rep Date: 2020-08-03 Impact factor: 4.379