Bioinspired DNA-Inorganic Hybrid Nanoflowers Combined with a Personal Glucose Meter for Onsite Detection of miRNA.

Biomineralization is an important process in nature, by which living organisms participate in producing organic/inorganic hybrid materials and the resultant materials show sophisticated structures and excellent physical and chemical properties. Inspired by biomineralization, DNA-Cu3(PO4)2 hybrid nanoflowers (HNFs) were prepared, which exhibited high stability, a high surface-to-volume ratio, and good DNA encapsulation ability. A facile thread platform for microRNA (miRNA) detection was fabricated by employing DNA-Cu3(PO4)2 HNFs as captors, and the signal could be easily read out by a personal glucose meter. The fabricated biosensor could detect miRNA-21 quantitatively and a detection limit of 0.41 nM was achieved. Furthermore, miRNA in A549 cell lysate could also be detected without pretreatment. In this work, we achieved a fast, simple, low-cost method based on the bioinspired DNA-inorganic HNFs for the specific and sensitive detection of miRNA in both aqueous solution and biological samples, indicating its great promise in biomedical and clinical applications.