| Literature DB >> 30455464 |
Rui Lin1,2, Ruiyu Wang1,2, Jing Yuan3,4,5, Qiru Feng2,6, Youtong Zhou2, Shaoqun Zeng3,4, Miao Ren3,4, Siqi Jiang3,4, Hong Ni3,4, Can Zhou3,4, Hui Gong7,8,9, Minmin Luo10,11,12.
Abstract
We developed a dual-adeno-associated-virus expression system that enables strong and sparse labeling of individual neurons with cell-type and projection specificity. We demonstrated its utility for whole-brain reconstruction of midbrain dopamine neurons and striatum-projecting cortical neurons. We further extended the labeling method for rapid reconstruction in cleared thick brain sections and simultaneous dual-color labeling. This labeling system may facilitate the process of generating mesoscale single-neuron projectomes of mammalian brains.Entities:
Mesh：
Year: 2018 PMID： 30455464 DOI： 10.1038/s41592-018-0184-y
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547