Karin de Ruiter1, Dicky L Tahapary1,2, Erliyani Sartono1, Thomas B Nutman3, Johannes W A Smit4,5, Leo Koenderman6,7, Taniawati Supali8, Maria Yazdanbakhsh1. 1. Department of Parasitology, Leiden University Medical Center, The Netherlands. 2. Department of Internal Medicine, Division of Endocrinology and Metabolism, Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia. 3. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland. 4. Department of Internal Medicine, Leiden University Medical Center, The Netherlands. 5. Department of Internal Medicine, Radboud University Medical Centre, Nijmegen, The Netherlands. 6. Laboratory of Translational Immunology, University Medical Center Utrecht, The Netherlands. 7. Department of Respiratory Medicine, University Medical Center Utrecht, The Netherlands. 8. Department of Parasitology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Abstract
BACKGROUND: Eosinophils are a prominent cell type in the host response to helminths, and some evidence suggests that neutrophils might also play a role. However, little is known about the activation status of these granulocytes during helminth infection. METHODS: We analyzed the expression of eosinophil and neutrophil activation markers in peripheral blood by flow cytometry and measured serum levels of eosinophil granule proteins in 300 subjects residing in an area endemic for soil-transmitted helminths (STH). The data generated are on samples before and after 1 year of 3-monthly albendazole treatment. RESULTS: Anthelmintic treatment significantly reduced the prevalence of STH. While eosinophil numbers were significantly higher in STH-infected compared to uninfected subjects and significantly decreased following albendazole treatment, there was no effect exerted by the helminths on either eosinophil nor neutrophil activation. Although at baseline eosinophil granule protein levels were not different between STH-infected and uninfected subjects, treatment significantly reduced the levels of eosinophil-derived neurotoxin (EDN) in those infected at baseline. CONCLUSIONS: These results show that besides decreasing eosinophil numbers, anthelmintic treatment does not significantly change the activation status of eosinophils, nor of neutrophils, and the only effect seen was a reduction in circulating levels of EDN. CLINICAL TRIALS REGISTRATION: http://www.isrctn.com/ISRCTN75636394.
RCT Entities:
BACKGROUND: Eosinophils are a prominent cell type in the host response to helminths, and some evidence suggests that neutrophils might also play a role. However, little is known about the activation status of these granulocytes during helminth infection. METHODS: We analyzed the expression of eosinophil and neutrophil activation markers in peripheral blood by flow cytometry and measured serum levels of eosinophil granule proteins in 300 subjects residing in an area endemic for soil-transmitted helminths (STH). The data generated are on samples before and after 1 year of 3-monthly albendazole treatment. RESULTS: Anthelmintic treatment significantly reduced the prevalence of STH. While eosinophil numbers were significantly higher in STH-infected compared to uninfected subjects and significantly decreased following albendazole treatment, there was no effect exerted by the helminths on either eosinophil nor neutrophil activation. Although at baseline eosinophil granule protein levels were not different between STH-infected and uninfected subjects, treatment significantly reduced the levels of eosinophil-derived neurotoxin (EDN) in those infected at baseline. CONCLUSIONS: These results show that besides decreasing eosinophil numbers, anthelmintic treatment does not significantly change the activation status of eosinophils, nor of neutrophils, and the only effect seen was a reduction in circulating levels of EDN. CLINICAL TRIALS REGISTRATION: http://www.isrctn.com/ISRCTN75636394.
Authors: Dicky L Tahapary; Karin de Ruiter; Ivonne Martin; Eric A T Brienen; Lisette van Lieshout; Christa M Cobbaert; Pradana Soewondo; Yenny Djuardi; Aprilianto E Wiria; Jeanine J Houwing-Duistermaat; Erliyani Sartono; Johannes W A Smit; Maria Yazdanbakhsh; Taniawati Supali Journal: Clin Infect Dis Date: 2017-09-01 Impact factor: 9.079
Authors: Pedro H Gazzinelli-Guimarães; Sandra Bonne-Année; Ricardo T Fujiwara; Helton C Santiago; Thomas B Nutman Journal: J Immunol Date: 2016-08-26 Impact factor: 5.422
Authors: Dicky L Tahapary; Karin de Ruiter; Ivonne Martin; Lisette van Lieshout; Bruno Guigas; Pradana Soewondo; Yenny Djuardi; Aprilianto E Wiria; Oleg A Mayboroda; Jeanine J Houwing-Duistermaat; Hengki Tasman; Erliyani Sartono; Maria Yazdanbakhsh; Johannes W A Smit; Taniawati Supali Journal: BMC Infect Dis Date: 2015-03-18 Impact factor: 3.090