Literature DB >> 30451431

Molecular Identification of Entomopathogenic Nematode Isolates from the Philippines and their Biological Control Potential Against Lepidopteran Pests of Corn.

Barbara L Caoili1, Romnick A Latina1, Regina Faye C Sandoval1, Joey I Orajay2.   

Abstract

In search for local entomopathogenic nematode (EPN) species as a biological control agent of lepidopterous insect pests of corn, a survey for EPN in the major islands in the Philippines was conducted. Seven EPN populations from 279 soil samples were isolated using Ostrinia furnacalis, the key target insect pest of corn in the country, as bait. Analysis of the ITS1-5.8S-ITS2 ribosomal DNA sequence revealed the presence of Steinernema abbasi, Steinernema minutum , Steinernema tami , and Heterorhabditis indica . The pathogenicity of these EPN was tested in Ostrinia furnacalis , Spodoptera litura , and Helicoverpa armigera larvae under laboratory conditions. All the EPN isolates were pathogenic to the lepidopteran species with, H. indica PBCB and S. abbasi MBLB exhibiting the highest virulence (88%-99.33% and 90%-100% mortality, respectively) at 48 hr post infection (HPI) and thus, further studies were done on these two EPN. The highest penetration rate at 48 HPI was observed in H. armigera infected with S. abbasi MBLB (28.15%), while the lowest was in O. furnacalis infected with H. indica PBCB (14.25%). Nonetheless, based on LC 50 at 48 HPI, H. indica PBCB was most virulent to S. litura (8.89 IJ per larva), but not significantly different from O. furnacalis (10.52 IJ per larva). Steinernema abbasi MBLB was most virulent to O. furnacalis (10.98 IJ per larva), but not significantly different to S. litura (17.08 IJ per larva). LT 50 estimates showed that O. furnacalis was significantly the most susceptible to H. indica PBCB (21.90 hr) and S. abbasi (21.18 hr). Our results suggest that H. indica PBCB and S. abbasi MBLB are good candidates as biological control agents against these insect pests of corn. Moreover, O. furnacalis as alternative bait for EPN was discussed. To date, this is the most extensive research on Philippine EPN, comprised of wide sampling coverage, molecular identification and bioefficacy assays. © The Society of Nematologists.

Entities:  

Keywords:  Biological control; Entomopathogenic nematode; Helicoverpa armigera; Heterorhabditis indica; Internal transcribed spacer; Ostrinia furnacalis; Ribosomal DNA region; Spodoptera litura; Steinernema abbasi

Year:  2018        PMID: 30451431      PMCID: PMC6909345          DOI: 10.21307/jofnem-2018-024

Source DB:  PubMed          Journal:  J Nematol        ISSN: 0022-300X            Impact factor:   1.402


  21 in total

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2.  Entomopathogenic nematodes for the biological control of insects.

Authors:  G C Smart
Journal:  J Nematol       Date:  1995-12       Impact factor: 1.402

Review 3.  Insect immune responses to nematode parasites.

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Authors:  Kathryn A Barbara; Eileen A Buss
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6.  Steinernema brazilense n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Mato Grosso, Brazil.

Authors:  Khuong B Nguyen; Carmen M Ambrós Ginarte; Luís G Leite; Jaime M dos Santos; Ricardo Harakava
Journal:  J Invertebr Pathol       Date:  2009-09-20       Impact factor: 2.841

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Authors:  Hari Bahadur Khatri-Chhetri; Lieven Waeyenberge; Hira Kaji Manandhar; Maurice Moens
Journal:  J Invertebr Pathol       Date:  2009-10-28       Impact factor: 2.841

8.  Asian corn borer (ACB) and non-ACB pests in GM corn (Zea mays L.) in the Philippines.

Authors:  Miladis M Afidchao; C J M Musters; Geert R de Snoo
Journal:  Pest Manag Sci       Date:  2013-02-08       Impact factor: 4.845

Review 9.  Insect resistance to Bt crops: lessons from the first billion acres.

Authors:  Bruce E Tabashnik; Thierry Brévault; Yves Carrière
Journal:  Nat Biotechnol       Date:  2013-06       Impact factor: 54.908

10.  ABCs of Insect Resistance to Bt.

Authors:  Bruce E Tabashnik
Journal:  PLoS Genet       Date:  2015-11-19       Impact factor: 5.917

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Authors:  Wei-Ting Liu; Tien-Lai Chen; Roger F Hou; Cheng-Chen Chen; Wu-Chun Tu
Journal:  Insects       Date:  2020-11-26       Impact factor: 2.769

2.  A study on Xenorhabdus and Photorhabdus isolates from Northeastern Thailand: Identification, antibacterial activity, and association with entomopathogenic nematode hosts.

Authors:  Thatcha Yimthin; Chamaiporn Fukruksa; Paramaporn Muangpat; Abdulhakam Dumidae; Wandee Wattanachaiyingcharoen; Apichat Vitta; Aunchalee Thanwisai
Journal:  PLoS One       Date:  2021-08-12       Impact factor: 3.240

  2 in total

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