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Literature DB >> 3045097

Purification of Rts1 RepA protein and binding of the protein to mini-Rts1 DNA.

Abstract

RepA protein, essential for the replication of plasmid Rts1, was purified, and its binding to mini-Rts1 subregions was examined by a DNase I protection assay. RepA protected the incI and incII iterons, a region immediately upstream of the repA promoter, and a 10-base-pair region located between the most external incII iteron and a GATC box. The protection was less efficient when preheated RepA was used.

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Year: 1988 PMID： 3045097 PMCID： PMC211463 DOI： 10.1128/jb.170.9.4411-4414.1988

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

20 in total

1. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.

Authors: D J Galas; A Schmitz
Journal: Nucleic Acids Res Date: 1978-09 Impact factor: 16.971

2. Nucleotide sequence and copy control function of the extension of the incI region (incI-b) of Rts 1.

Authors: H Nozue; K Tsuchiya; Y Kamio
Journal: Plasmid Date: 1988-01 Impact factor: 3.466

3. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

4. Replication of the R factor Rts1 in Proteus mirabilis.

Authors: Y Terawaki; R Rownd
Journal: J Bacteriol Date: 1972-02 Impact factor: 3.490

5. Purification and properties of the mini-F plasmid-encoded E protein needed for autonomous replication control of the plasmid.

Authors: T Tokino; T Murotsu; K Matsubara
Journal: Proc Natl Acad Sci U S A Date: 1986-06 Impact factor: 11.205

6. Cloning of the replication and incompatibility regions of a plasmid derived from Rts1.

Authors: Y Itoh; Y Kamio; Y Furuta; Y Terawaki
Journal: Plasmid Date: 1982-11 Impact factor: 3.466

7. Purification of bacteriophage lambda O protein that specifically binds to the origin of replication.

Authors: T Tsurimoto; K Matsubara
Journal: Mol Gen Genet Date: 1981

8. A system for shotgun DNA sequencing.

Authors: J Messing; R Crea; P H Seeburg
Journal: Nucleic Acids Res Date: 1981-01-24 Impact factor: 16.971

9. Importance of the C terminus of plasmid Rts1 RepA protein for replication and incompatibility of the plasmid.

Authors: Y Terawaki; Z Hong; Y Itoh; Y Kamio
Journal: J Bacteriol Date: 1988-03 Impact factor: 3.490

10. Two functions of the E protein are key elements in the plasmid F replication control system.

Authors: L A Rokeach; L Søgaard-Andersen; S Molin
Journal: J Bacteriol Date: 1985-12 Impact factor: 3.490

10 in total

1. Protein-DNA interactions in regulation of P1 plasmid replication.

Authors: A L Abeles; L D Reaves; S J Austin
Journal: J Bacteriol Date: 1989-01 Impact factor: 3.490

2. Nucleotide sequence analysis and expression of the minimum REPI replication region and incompatibility determinants of pColV-K30.

Authors: J F Perez-Casal; A E Gammie; J H Crosa
Journal: J Bacteriol Date: 1989-04 Impact factor: 3.490

3. Effects of mutations in the repA gene of plasmid Rts1 on plasmid replication and autorepressor function.

Authors: Y Terawaki; H Nozue; H Zeng; T Hayashi; Y Kamio; Y Itoh
Journal: J Bacteriol Date: 1990-02 Impact factor: 3.490

4. Functional characterization of a replication initiator protein.

Authors: A E Gammie; M E Tolmasky; J H Crosa
Journal: J Bacteriol Date: 1993-06 Impact factor: 3.490

5. An unusual mutation in RepA increases the copy number of a stringently controlled plasmid (Rts1 derivative) by over one hundred fold.

Authors: H Yonemitsu; H Higuchi; T Fujihashi; A Kaji
Journal: Mol Gen Genet Date: 1995-02-06

6. Cooperative binding of initiator protein to replication origin conferred by single amino acid substitution.

Authors: M Filutowicz; D York; I Levchenko
Journal: Nucleic Acids Res Date: 1994-10-11 Impact factor: 16.971

7. Site-directed mutations in the repA C-terminal region of plasmid Rts1: pleiotropic effects on the replication and autorepressor functions.

Authors: H Zeng; T Hayashi; Y Terawaki
Journal: J Bacteriol Date: 1990-05 Impact factor: 3.490