Literature DB >> 30433

Metal chelate affinity chromatography of hamster interferon.

E Bollin, E Sulkowski.   

Abstract

Syrian hamster interferon, produced by benzo(a)pyrene-transformed embryo cells does not display an affinity for Zn++ chelate-agarose. However, the interferon binds to Cu++ chelate-agarose and can be displaced from the column by a falling pH-gradient. The chromatography of crude hamster interferon preparation on a tandem of columsn: Zn++ chelate-agarose in equilibrium Cu++ chelate-agarose results in a 25-fold purification of interferon.

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Year:  1978        PMID： 30433     DOI： 10.1007/BF01315407

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  8 in total

1.  Metal chelate affinity chromatography, a new approach to protein fractionation.

Authors:  J Porath; J Carlsson; I Olsson; G Belfrage
Journal:  Nature       Date:  1975-12-18       Impact factor: 49.962

2.  Purification of human fibroblast interferon by zinc chelate affinity chromatography.

Authors:  V G Edy; A Billiau; P de Somer
Journal:  J Biol Chem       Date:  1977-09-10       Impact factor: 5.157

3.  Isolation of lactoferrin from human milk by metal-chelate affinity chromatography.

Authors:  J Carlsson; J Porath; B Lönnerdal
Journal:  FEBS Lett       Date:  1977-03-15       Impact factor: 4.124

4.  Fluorometric assay of proteins in the nanogram range.

Authors:  P Böhlen; S Stein; W Dairman; S Udenfriend
Journal:  Arch Biochem Biophys       Date:  1973-03       Impact factor: 4.013

5.  Structural requirements of the rI n -rC n complex for induction of human interferon.

Authors:  W A Carter; P M Pitha; L W Marshall; I Tazawa; S Tazawa; P O Ts'o
Journal:  J Mol Biol       Date:  1972-10-14       Impact factor: 5.469

6.  Purification of the human plasma alpha2-SH glycoprotein by zinc chelate affinity chromatography.

Authors:  J P Lebreton
Journal:  FEBS Lett       Date:  1977-08-15       Impact factor: 4.124

7.  The configuration of cobaltodihistidine and oxy-bis (cobaltodihistidine).

Authors:  J Z HEARON
Journal:  J Natl Cancer Inst       Date:  1948-08       Impact factor: 13.506

8.  Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells.

Authors:  R L Cavalieri; E A Havell; J Vilcek; S Pestka
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205
  8 in total
  1 in total

1.  Protein selectivity in immobilized metal affinity chromatography based on the surface accessibility of aspartic and glutamic acid residues.

Authors:  M Zachariou; M T Hearn
Journal:  J Protein Chem       Date:  1995-08
  1 in total

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