| Literature DB >> 30431032 |
Jin Jiao1, Tao Gao, Hai Shi, Anzhi Sheng, Yang Xiang, Yongqian Shu, Genxi Li.
Abstract
A method to directly assay circular RNA (circRNA) is proposed in this work by utilizing the 'microRNA (miRNA) sponge' nature of circRNA and by taking the advantage of duplex-specific nuclease. Moreover, miRNA absorption site-mediated hairpin DNA unfolding and nuclease-assisted target recycling have also been designed in this method to amplify the signal readout, thus sensitive assay of circRNA can be achieved in real samples.Mesh：
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Year: 2018 PMID： 30431032 DOI： 10.1039/c8cc08319c
Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222