Carlos Hg Martins1, Fariza Abrão1, Thaís S Moraes1, Pollyanna F Oliveira2, Denise C Tavares2, Lizandra G Magalhães3, Fábio C Galvão4, Rodrigo Cs Veneziani3, Sérgio R Ambrósio3. 1. Research Laboratory of Applied Microbiology, University of Franca - UNIFRAN, Franca, 14404-600, São Paulo, Brazil. 2. Laboratory of Mutagenesis, University of Franca - UNIFRAN, Franca, 14404-600, São Paulo, Brazil. 3. Nucleus of Research in Sciences & Technology, University of Franca - UNIFRAN, 14404-600, São Paulo, Brazil. 4. Department of Biological Sciences, São Paulo State University - UNESP, Araraquara, 14800-901, São Paulo, Brazil.
Abstract
AIM: To evaluate the antibacterial activity of 12 kaurane-type diterpenes against a panel of bacteria that cause endodontic infection. METHODS & MATERIALS: We conducted tests against bacteria in the planktonic or in the sessile mode, cytotoxic assays for the most promising compounds against human normal lung fibroblast cells, and Porphyromonas gingivalis (ATCC 33277) proteomic analysis. RESULTS & CONCLUSION: Kaurenoic acid and its salt exhibited satisfactory antibacterial action against the evaluated bacteria. Proteomic analysis suggested that these compounds might interfere in bacterial metabolism and virulence factor expression. Kaurane-type diterpenes are an important class of natural products and should be considered in the search for new irrigating solutions to treat endodontic infections.
AIM: To evaluate the antibacterial activity of 12 kaurane-type diterpenes against a panel of bacteria that cause endodontic infection. METHODS & MATERIALS: We conducted tests against bacteria in the planktonic or in the sessile mode, cytotoxic assays for the most promising compounds against human normal lung fibroblast cells, and Porphyromonas gingivalis (ATCC 33277) proteomic analysis. RESULTS & CONCLUSION:Kaurenoic acid and its salt exhibited satisfactory antibacterial action against the evaluated bacteria. Proteomic analysis suggested that these compounds might interfere in bacterial metabolism and virulence factor expression. Kaurane-type diterpenes are an important class of natural products and should be considered in the search for new irrigating solutions to treat endodontic infections.