Linda Rose Jose1, Sumana Neelambike Mahadeviah1, V Balamurugan2, K Ramachandra Kini3. 1. Department of Microbiology, JSS Medical College, Mysore, Karnataka, India. 2. Department of Microbiology, ICAR-NIVEDI, Bengaluru, Karnataka, India. 3. Department of Biotechnology, University of Mysore, Mysore, Karnataka, India.
Abstract
BACKGROUND: Leptospirosis is a zoonotic disease of ubiquitous distribution. During rainy seasons, in spring and summer and also during harvest times, the risk of leptospirosis increases as there are chances of frequent contact with infected rat population which is common in Karnataka as farming is a main source of income to the people here. There is a paucity of data regarding the prevalent serovars from Karnataka. This study was undertaken as an attempt to compare a battery of tools such as immunochromatographic test (ICT), microscopic agglutination test (MAT), immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) and in-house polymerase chain reaction (PCR) to detect leptospirosis. SETTINGS AND DESIGN: This study using consecutive sampling technique was conducted in a tertiary care centre, Mysore, Karnataka. SUBJECTS AND METHODS: Samples from 783 suspected cases of leptospirosis in and around Mysore between April 2013 and April 2016 were processed. Samples from 783 patients suspected of leptospirosis were subjected to ICT, IgM ELISA, MAT and in-house PCR. STATISTICAL ANALYSIS USED: The statistical analysis was carried out using SPSS software version. RESULTS: Among 783 samples tested, only 14 (1.7%) were positive by ICT, 341 (44%) were positive by IgM ELISA, 368 (47%) were positive by MAT and 393 (50.2%) were positive by in-house PCR. CONCLUSIONS: Mysore can be considered endemic for leptospirosis. The in-house PCR based on LipL32 gene proved to be useful in the early diagnosis of leptospirosis.
BACKGROUND: Leptospirosis is a zoonotic disease of ubiquitous distribution. During rainy seasons, in spring and summer and also during harvest times, the risk of leptospirosis increases as there are chances of frequent contact with infected rat population which is common in Karnataka as farming is a main source of income to the people here. There is a paucity of data regarding the prevalent serovars from Karnataka. This study was undertaken as an attempt to compare a battery of tools such as immunochromatographic test (ICT), microscopic agglutination test (MAT), immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) and in-house polymerase chain reaction (PCR) to detect leptospirosis. SETTINGS AND DESIGN: This study using consecutive sampling technique was conducted in a tertiary care centre, Mysore, Karnataka. SUBJECTS AND METHODS: Samples from 783 suspected cases of leptospirosis in and around Mysore between April 2013 and April 2016 were processed. Samples from 783 patients suspected of leptospirosis were subjected to ICT, IgM ELISA, MAT and in-house PCR. STATISTICAL ANALYSIS USED: The statistical analysis was carried out using SPSS software version. RESULTS: Among 783 samples tested, only 14 (1.7%) were positive by ICT, 341 (44%) were positive by IgM ELISA, 368 (47%) were positive by MAT and 393 (50.2%) were positive by in-house PCR. CONCLUSIONS: Mysore can be considered endemic for leptospirosis. The in-house PCR based on LipL32 gene proved to be useful in the early diagnosis of leptospirosis.
Entities:
Keywords:
Immunochromatographic test; LipL 32 polymerase chain reaction; immunoglobulin M enzyme-linked immunosorbent assay; leptospirosis; microscopic agglutination test
Authors: Andrei E Samoilov; Nataliya A Stoyanova; Nikolai K Tokarevich; Birgitta Evengard; Elena V Zueva; Yulia A Panferova; Yulia V Ostankova; Elena B Zueva; Diana E Valutite; Evgeniy V Kovalev; Anna R Litovko; Alexey U Goncharov; Alexandr V Semenov; Kamil Khafizov; Vladimir G Dedkov Journal: Int J Environ Res Public Health Date: 2020-06-14 Impact factor: 3.390