Literature DB >> 30427336

A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases.

S Leichnitz1, J Heinrich, N Kulak.   

Abstract

Metal complexes can initiate DNA cleavage by the formation of reactive oxygen species (ROS). A conventional assay to probe for ROS is to add quenchers in a gel electrophoresis experiment. As we show here, such an assay is neither selective nor reliable. Instead, we suggest the use of simple fluorogens, as tested here with several metallonucleases for the detection of H2O2 and HO˙.

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Year:  2018        PMID： 30427336     DOI： 10.1039/c8cc06996d

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  7 in total

1.  Precision targeting of bacterial pathogen via bi-functional nanozyme activated by biofilm microenvironment.

Authors:  Yue Huang; Yuan Liu; Shrey Shah; Dongyeop Kim; Aurea Simon-Soro; Tatsuro Ito; Maryam Hajfathalian; Yong Li; Jessica C Hsu; Lenitza M Nieves; Faizan Alawi; Pratap C Naha; David P Cormode; Hyun Koo
Journal:  Biomaterials       Date:  2020-11-27       Impact factor: 12.479

2.  Incorporation of β-Alanine in Cu(II) ATCUN Peptide Complexes Increases ROS Levels, DNA Cleavage and Antiproliferative Activity.

Authors:  Julian Heinrich; Karolina Bossak-Ahmad; Mie Riisom; Haleh H Haeri; Tasha R Steel; Vinja Hergl; Alexander Langhans; Corinna Schattschneider; Jannis Barrera; Stephen M F Jamieson; Matthias Stein; Dariush Hinderberger; Christian G Hartinger; Wojciech Bal; Nora Kulak
Journal:  Chemistry       Date:  2021-12-04       Impact factor: 5.020

3.  Antibacterial Nanoparticles with Natural Photosensitizers Extracted from Spinach Leaves.

Authors:  Pavel Ludačka; Pavel Kubát; Zuzana Bosáková; Jiří Mosinger
Journal:  ACS Omega       Date:  2021-12-17

Review 4.  Nuclease-like metalloscissors: Biomimetic candidates for cancer and bacterial and viral infections therapy.

Authors:  Marzieh Anjomshoa; Bagher Amirheidari
Journal:  Coord Chem Rev       Date:  2022-02-05       Impact factor: 22.315

5.  A magnetically separable and recyclable g-C3N4/Fe3O4/porous ruthenium nanocatalyst for the photocatalytic degradation of water-soluble aromatic amines and azo dyes.

Authors:  Anupam Sahoo; Srikanta Patra
Journal:  RSC Adv       Date:  2020-02-06       Impact factor: 4.036

6.  Investigating Alkylated Prodigiosenes and Their Cu(II)-Dependent Biological Activity: Interactions with DNA, Antimicrobial and Photoinduced Anticancer Activity.

Authors:  Sebastian Doniz Kettenmann; Matthew White; Julien Colard-Thomas; Matilda Kraft; Andrea T Feßler; Karin Danz; Gerhard Wieland; Sylvia Wagner; Stefan Schwarz; Arno Wiehe; Nora Kulak
Journal:  ChemMedChem       Date:  2021-12-22       Impact factor: 3.540

Review 7.  Photo-Oxidation of Therapeutic Protein Formulations: From Radical Formation to Analytical Techniques.

Authors:  Elena Hipper; Michaela Blech; Dariush Hinderberger; Patrick Garidel; Wolfgang Kaiser
Journal:  Pharmaceutics       Date:  2021-12-28       Impact factor: 6.321
  7 in total

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