Jiro Sakamoto1, Kazuyoshi Shigehara2, Kazufumi Nakashima1, Shohei Kawaguchi1, Takao Nakashima3, Masayoshi Shimamura4, Mitsuru Yasuda5, Taku Kato5, Toru Hasegawa6, Yoshitomo Kobori7, Hiroshi Okada7, Takashi Deguchi5, Kouji Izumi1, Yoshifumi Kadono1, Atsushi Mizokami1. 1. Department of Urology, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa, Japan. 2. Department of Urology, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa, Japan. Electronic address: kshigehara0415@yahoo.co.jp. 3. Department of Urology, Ishikawa Prefectural Central Hospital, Kanazawa, Ishikawa, Japan. 4. Department of Urology, Nomi City Hospital, Nomi, Ishikawa, Japan. 5. Department of Urology, Gifu University Hospital, Gifu, Gifu, Japan. 6. Department of Urology, Hasegawa Hospital, Japan. 7. Department of Urology, Dokkyo Medical School Koshigaya Hospital, Japan.
Abstract
OBJECTIVE: To examine the association between human papillomavirus (HPV) infection and penile cancer among Japanese patients. METHODS: Thirty-four patients with penile cancer were enrolled in this study. DNA was extracted from paraffin-embedded tumor tissue samples, and HPV-DNA tests and genotyping were performed. For all of the samples, in situ hybridization (ISH) was performed to locate HPV-DNA in tumor tissue. Furthermore, expression levels of p16-INK4a, mini-chromosome maintenance protein 7(mcm-7), HPV-L1, and Ki-67 were analyzed using immunohistochemical methods. RESULTS: HPV and high-risk (HR)-HPV were detected in 14 (41.1%; 95% confidence interval (CI) 24.6-57.7%) and 12 (35.2%; 95% CI 19.2-51.4%) cases, respectively. HPV16 was the most frequently detected HPV type. Among the HR-HPV-positive cases, a punctate HR-HPV-DNA signal pattern was detected by ISH in tumor cell nuclei. P16-INK4a was expressed in 66.7% (95% CI 42.8-90.1%) of HR-HPV-positive cases and was significantly more frequent and stronger in HR-HPV-positive cases than in HPV-negative cases. There was no significant difference in the occurrence or distribution of mcm-7 or Ki-67 expression between HPV-positive and HPV-negative cases. HPV-L1 expression was not observed in any of the cases examined. CONCLUSIONS: HPV infection may have had an etiological role in 41% of the examined cases of penile cancer in Japan.
OBJECTIVE: To examine the association between human papillomavirus (HPV) infection and penile cancer among Japanese patients. METHODS: Thirty-four patients with penile cancer were enrolled in this study. DNA was extracted from paraffin-embedded tumor tissue samples, and HPV-DNA tests and genotyping were performed. For all of the samples, in situ hybridization (ISH) was performed to locate HPV-DNA in tumor tissue. Furthermore, expression levels of p16-INK4a, mini-chromosome maintenance protein 7(mcm-7), HPV-L1, and Ki-67 were analyzed using immunohistochemical methods. RESULTS:HPV and high-risk (HR)-HPV were detected in 14 (41.1%; 95% confidence interval (CI) 24.6-57.7%) and 12 (35.2%; 95% CI 19.2-51.4%) cases, respectively. HPV16 was the most frequently detected HPV type. Among the HR-HPV-positive cases, a punctate HR-HPV-DNA signal pattern was detected by ISH in tumor cell nuclei. P16-INK4a was expressed in 66.7% (95% CI 42.8-90.1%) of HR-HPV-positive cases and was significantly more frequent and stronger in HR-HPV-positive cases than in HPV-negative cases. There was no significant difference in the occurrence or distribution of mcm-7 or Ki-67 expression between HPV-positive and HPV-negative cases. HPV-L1 expression was not observed in any of the cases examined. CONCLUSIONS:HPV infection may have had an etiological role in 41% of the examined cases of penile cancer in Japan.
Authors: Anna Sophie Ramsauer; Garrett Louis Wachoski-Dark; Cornel Fraefel; Kurt Tobler; Sabine Brandt; Cameron Greig Knight; Claude Favrot; Paula Grest Journal: BMC Vet Res Date: 2019-10-22 Impact factor: 2.741