Mubashir Hassan1, Saba Shahzadi2,3, Hussain Raza1, Muhammad Athar Abbasi1,4, Hany Alashwal5, Nazar Zaki5, Ahmed A Moustafa6,7,8, Sung-Yum Seo1. 1. a Department of Biological Sciences, College of Natural Sciences , Kongju National University , Gongju , Chungnam-do 32588 , Republic of Korea. 2. b Institute of Molecular Science and Bioinformatics , Lahore , Pakistan. 3. c Department of Bioinformatics , Virtual University of Pakistan , Lahore , Pakistan. 4. d Department of Chemistry , Government College University , Lahore , Pakistan. 5. e Department of Computer Science and Software Engineering, College of Information Technology , United Arab Emirates University , Al-Ain , United Arab Emirates. 6. f School of Social Sciences and Psychology. 7. g MARCS Institute for Brain and Behaviour , Western Sydney University , Sydney , New South Wales , Australia. 8. h Department of Social Sciences, College of Arts and Sciences , Qatar University , Doha , Qatar'.
Abstract
AIM: Amyloid beta (Aβ) 1-42, which is a basic constituent of amyloid plaques, binds with extracellular transmembrane receptor nicotine acetylcholine receptor α7 (nAChRα7) in Alzheimer's disease. MATERIALS AND METHODS: In the current study, a computational approach was employed to explore the active binding sites of nAChRα7 through Aβ 1-42 interactions and their involvement in the activation of downstream signalling pathways. Sequential and structural analyses were performed on the extracellular part of nAChRα7 to identify its core active binding site. RESULTS: Results showed that a conserved residual pattern and well superimposed structures were observed in all nAChRs proteins. Molecular docking servers were used to predict the common interactive residues in nAChRα7 and Aβ1-42 proteins. The docking profile results showed some common interactive residues such as Glu22, Ala42 and Trp171 may consider as the active key player in the activation of downstream signalling pathways. Moreover, the signal communication and receiving efficacy of best-docked complexes was checked through DynOmic online server. Furthermore, the results from molecular dynamic simulation experiment showed the stability of nAChRα7. The generated root mean square deviations and fluctuations (RMSD/F), solvent accessible surface area (SASA) and radius of gyration (Rg) graphs of nAChRα7 also showed its backbone stability and compactness, respectively. CONCLUSION: Taken together, our predicted results intimated the structural insight on the molecular interactions of beta amyloid protein involved in the activation of nAChRα7 receptor. In future, a better understanding of nAChRα7 and their interconnected proteins signalling cascade may be consider as target to cure Alzheimer's disease.
AIM: Amyloid beta (Aβ) 1-42, which is a basic constituent of amyloid plaques, binds with extracellular transmembrane receptor nicotineacetylcholine receptor α7 (nAChRα7) in Alzheimer's disease. MATERIALS AND METHODS: In the current study, a computational approach was employed to explore the active binding sites of nAChRα7 through Aβ 1-42 interactions and their involvement in the activation of downstream signalling pathways. Sequential and structural analyses were performed on the extracellular part of nAChRα7 to identify its core active binding site. RESULTS: Results showed that a conserved residual pattern and well superimposed structures were observed in all nAChRs proteins. Molecular docking servers were used to predict the common interactive residues in nAChRα7 and Aβ1-42 proteins. The docking profile results showed some common interactive residues such as Glu22, Ala42 and Trp171 may consider as the active key player in the activation of downstream signalling pathways. Moreover, the signal communication and receiving efficacy of best-docked complexes was checked through DynOmic online server. Furthermore, the results from molecular dynamic simulation experiment showed the stability of nAChRα7. The generated root mean square deviations and fluctuations (RMSD/F), solvent accessible surface area (SASA) and radius of gyration (Rg) graphs of nAChRα7 also showed its backbone stability and compactness, respectively. CONCLUSION: Taken together, our predicted results intimated the structural insight on the molecular interactions of beta amyloid protein involved in the activation of nAChRα7 receptor. In future, a better understanding of nAChRα7 and their interconnected proteins signalling cascade may be consider as target to cure Alzheimer's disease.