Juliano H Borges1, Gil Guerra-Júnior2, Ezequiel M Gonçalves3. 1. Growth and Development Laboratory, Center for Investigation in Pediatrics, School of Medicine, University of Campinas, Campinas, São Paulo, Brazil. Electronic address: borges02@hotmail.com. 2. Growth and Development Laboratory, Center for Investigation in Pediatrics, School of Medicine, University of Campinas, Campinas, São Paulo, Brazil; Department of Pediatrics, University of Campinas, Campinas, São Paulo, Brazil. 3. Growth and Development Laboratory, Center for Investigation in Pediatrics, School of Medicine, University of Campinas, Campinas, São Paulo, Brazil.
Abstract
OBJECTIVES: The aim of this study was to test the accuracy of different methods of resting energy expenditure (REE) data analysis using indirect calorimetry (IC) during traditional (30 min) and abbreviated (10 min) protocols. METHODS: Fifteen women and 15 men (21-34 y of age) completed two consecutive 30-min IC measurements. Body composition was measured using dual-energy x-ray absorptiometry. The reference method for REE analysis was 5 min in steady state (SS) during 30 min (first 5 min discarded). REE measurements were randomized to define a reference or testing method. An interval method was defined using 25, 20, and 15 min (with first 5, 10, and 15 min discarded, respectively), during 30 min, and 5 min (first 5 min discarded) during 10-min intervals. The SS method was defined using 5 min in SS (first 5 min discarded) during 30 min, 5, 4, and 3 min in SS during 10-min (first 5 min discarded) intervals. RESULTS: Interval methods during 30 min and SS and interval methods during 10 min demonstrated large bias with significantly high REEs compared to the reference method (78.8-109.0 kcal/d, all P < 0.001). Testing methods demonstrated large upper limits of agreement between 225.2 and 322.8 kcal/d. No mean differences (P > 0.05), small bias (14.3 kcal/d), and narrow limits of agreement (-125.8 to 154.4 kcal/d) were observed between 5-min SS during 30 min and the reference method. CONCLUSIONS: All interval methods and SS methods during 10 min overestimated REE. We recommend using 5-min SS during 30 min. The measurement may be repeated until all participants achieve SS.
OBJECTIVES: The aim of this study was to test the accuracy of different methods of resting energy expenditure (REE) data analysis using indirect calorimetry (IC) during traditional (30 min) and abbreviated (10 min) protocols. METHODS: Fifteen women and 15 men (21-34 y of age) completed two consecutive 30-min IC measurements. Body composition was measured using dual-energy x-ray absorptiometry. The reference method for REE analysis was 5 min in steady state (SS) during 30 min (first 5 min discarded). REE measurements were randomized to define a reference or testing method. An interval method was defined using 25, 20, and 15 min (with first 5, 10, and 15 min discarded, respectively), during 30 min, and 5 min (first 5 min discarded) during 10-min intervals. The SS method was defined using 5 min in SS (first 5 min discarded) during 30 min, 5, 4, and 3 min in SS during 10-min (first 5 min discarded) intervals. RESULTS: Interval methods during 30 min and SS and interval methods during 10 min demonstrated large bias with significantly high REEs compared to the reference method (78.8-109.0 kcal/d, all P < 0.001). Testing methods demonstrated large upper limits of agreement between 225.2 and 322.8 kcal/d. No mean differences (P > 0.05), small bias (14.3 kcal/d), and narrow limits of agreement (-125.8 to 154.4 kcal/d) were observed between 5-min SS during 30 min and the reference method. CONCLUSIONS: All interval methods and SS methods during 10 min overestimated REE. We recommend using 5-min SS during 30 min. The measurement may be repeated until all participants achieve SS.
Authors: Cassandra C Derella; Anson M Blanks; Andy Nguyen; Jacob Looney; Matthew A Tucker; Jinhee Jeong; Paula Rodriguez-Miguelez; Jeffrey Thomas; Matthew Lyon; David M Pollock; Ryan A Harris Journal: Am J Physiol Endocrinol Metab Date: 2022-04-04 Impact factor: 5.900
Authors: Juan M A Alcantara; Guillermo Sanchez-Delgado; Francisco J Amaro-Gahete; Jose E Galgani; Jonatan R Ruiz Journal: Nutrients Date: 2020-02-14 Impact factor: 5.717