Rong Zeng1, Yi Lyu2, Guoqiao Zhang1, Tao Shou1, Kai Wang1, Heng Niu3, Xinmin Yan4. 1. Department of Oncology, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology Kunming, Yunnan, China. 2. Department of Anesthesiology, Yunnan Baoshan Anli Hospital Baoshan, Yunnan, China. 3. Department of Breast and Thyroid Surgery, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology Kunming, Yunnan, China. 4. Institute of Clinical and Basic Medicine Research, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology Kunming, Yunnan, China.
Abstract
OBJECTIVES: To investigate the positive effect of Th17 cells on the prognosis of patients with PTC and HT. METHODS: The expression of nuclear specific marker RORγt of Th17 cells in fresh and paraffin thyroid tissues and serum specimens were analyzed. Flow cytometry was used to detect the formation rates of Th17 cells (CD3+CD8-IL-17A+/CD3+CD8-%) at different time points after co-culture of thyroid papillary carcinoma cell line (TPC-1 and K1) and umbilical cord blood initial T lymphocytes. The protein expression of RORγt in T lymphocytes after co-culture was detected. Preoperative serum levels of Th17 (IL-17) cytokines were measured. RESULTS: The positive expression of RORγt in the tumor microenvironment of PTC patients with or without HT could inhibit the lymph node metastasis of the tumor. PTC cancer cells could induce initial T lymphocyte to differentiate into Th17 cells, and the K1 cell line with lymph node metastasis induced a higher proportion of RORγt protein than that in TPC-1 cell line without lymph node metastasis. In PTC patients with HT, serum IL-17 concentration was negatively correlated with lymph node metastasis in the central group. CONCLUSIONS: RORγt may play an anti-tumor role in reducing thyroid cell damage by reducing the thyroid autoimmune antibodies TPOAb and TGAb in the PTC population in Yunnan plateau region.
OBJECTIVES: To investigate the positive effect of Th17 cells on the prognosis of patients with PTC and HT. METHODS: The expression of nuclear specific marker RORγt of Th17 cells in fresh and paraffin thyroid tissues and serum specimens were analyzed. Flow cytometry was used to detect the formation rates of Th17 cells (CD3+CD8-IL-17A+/CD3+CD8-%) at different time points after co-culture of thyroid papillary carcinoma cell line (TPC-1 and K1) and umbilical cord blood initial T lymphocytes. The protein expression of RORγt in T lymphocytes after co-culture was detected. Preoperative serum levels of Th17 (IL-17) cytokines were measured. RESULTS: The positive expression of RORγt in the tumor microenvironment of PTC patients with or without HT could inhibit the lymph node metastasis of the tumor. PTC cancer cells could induce initial T lymphocyte to differentiate into Th17 cells, and the K1 cell line with lymph node metastasis induced a higher proportion of RORγt protein than that in TPC-1 cell line without lymph node metastasis. In PTC patients with HT, serum IL-17 concentration was negatively correlated with lymph node metastasis in the central group. CONCLUSIONS: RORγt may play an anti-tumor role in reducing thyroid cell damage by reducing the thyroid autoimmune antibodies TPOAb and TGAb in the PTC population in Yunnan plateau region.
Authors: R M Melillo; V Guarino; E Avilla; M R Galdiero; F Liotti; N Prevete; F W Rossi; F Basolo; C Ugolini; A de Paulis; M Santoro; G Marone Journal: Oncogene Date: 2010-08-23 Impact factor: 9.867
Authors: E Fiore; T Rago; F Latrofa; M A Provenzale; P Piaggi; A Delitala; M Scutari; F Basolo; G Di Coscio; L Grasso; A Pinchera; P Vitti Journal: Endocr Relat Cancer Date: 2011-07-01 Impact factor: 5.678
Authors: Christian S Hinrichs; Andrew Kaiser; Chrystal M Paulos; Lydie Cassard; Luis Sanchez-Perez; Bianca Heemskerk; Claudia Wrzesinski; Zachary A Borman; Pawel Muranski; Nicholas P Restifo Journal: Blood Date: 2009-05-26 Impact factor: 22.113
Authors: S Matsubayashi; K Kawai; Y Matsumoto; T Mukuta; T Morita; K Hirai; F Matsuzuka; K Kakudoh; K Kuma; H Tamai Journal: J Clin Endocrinol Metab Date: 1995-12 Impact factor: 5.958
Authors: Lucas Leite Cunha; Elaine Cristina Morari; Suely Nonogaki; Natassia Elena Bufalo; Ligia Vera Montalli da Assumpção; Fernando Augusto Soares; José Vassallo; Laura Sterian Ward Journal: Sci Rep Date: 2020-03-05 Impact factor: 4.379