Cigdem Atalayin1, Huseyin Tezel2, Zeynep Ergucu2, Nimet Unlu3, Guliz Armagan4, Taner Dagci5, Timur Kose6. 1. Department of Restorative Dentistry, Ege University School of Dentistry, 35100, Izmir, Turkey. dtcatalayin@gmail.com. 2. Department of Restorative Dentistry, Ege University School of Dentistry, 35100, Izmir, Turkey. 3. Department of Restorative Dentistry, Selcuk University School of Dentistry, Konya, Turkey. 4. Department of Biochemistry, Ege University Faculty of Pharmacy, Izmir, Turkey. 5. Department of Physiology, Ege University School of Medicine, Izmir, Turkey. 6. Department of Biostatistics and Medical Informatics, Ege University School of Medicine, Izmir, Turkey.
Abstract
OBJECTIVE: The aim of this in vitro study is to evaluate the effects of resveratrol (RES) addition on the cytotoxicity and microtensile bond strength (μTBS) of different adhesives. MATERIALS AND METHODS: Five self-etching adhesives (G-aenial Bond-GC, Optibond All in One-Kerr, Gluma Self Etch-Kulzer, Clearfil S3 Bond-Kuraray, and Nova Compo-B Plus-Imicryl) were tested. They were applied to L-929 cell culture by the extract method. In the test groups, 0.5 μM RES (Sigma-Aldrich) was added into the medium. Cell viability was assessed by MTT assay after 24 h. Human extracted third molars were used for μTBS test (n = 7). The adhesives with or without 0.5 μM RES addition were applied on dentin surfaces. A composite build-up was constructed. Then, the specimens were sectioned into multiple beams with the non-trimming version of the microtensile test and subjected to microtensile forces. Statistical analysis was performed using ANOVA and post hoc Tukey test (p ˂ 0.05). RESULTS: The extracts of all adhesives decreased the cell viability. However, RES addition increased the cell viability in all groups (p ˂ 0.05). RES addition did not cause any decrease in μTBS values of the adhesives compared to baseline. Optibond All in One showed the highest μTBS after RES addition. It was followed by Clerafil S3 Bond and Nova Compo-B Plus. No difference was determined between the Optibond All in One and Clearfil S3 Bond. There was difference between Optibond All in One and Nova Compo-B Plus (p ˂ 0.05). CONCLUSION: RES addition may improve the biocompatibility without causing negative influence on μTBS of the adhesives. CLINICAL RELEVANCE: RES addition has clinical applicable potential to overcome the adverse biocompatibility of adhesives.
OBJECTIVE: The aim of this in vitro study is to evaluate the effects of resveratrol (RES) addition on the cytotoxicity and microtensile bond strength (μTBS) of different adhesives. MATERIALS AND METHODS: Five self-etching adhesives (G-aenial Bond-GC, Optibond All in One-Kerr, Gluma Self Etch-Kulzer, Clearfil S3 Bond-Kuraray, and Nova Compo-B Plus-Imicryl) were tested. They were applied to L-929 cell culture by the extract method. In the test groups, 0.5 μM RES (Sigma-Aldrich) was added into the medium. Cell viability was assessed by MTT assay after 24 h. Human extracted third molars were used for μTBS test (n = 7). The adhesives with or without 0.5 μM RES addition were applied on dentin surfaces. A composite build-up was constructed. Then, the specimens were sectioned into multiple beams with the non-trimming version of the microtensile test and subjected to microtensile forces. Statistical analysis was performed using ANOVA and post hoc Tukey test (p ˂ 0.05). RESULTS: The extracts of all adhesives decreased the cell viability. However, RES addition increased the cell viability in all groups (p ˂ 0.05). RES addition did not cause any decrease in μTBS values of the adhesives compared to baseline. Optibond All in One showed the highest μTBS after RES addition. It was followed by Clerafil S3 Bond and Nova Compo-B Plus. No difference was determined between the Optibond All in One and Clearfil S3 Bond. There was difference between Optibond All in One and Nova Compo-B Plus (p ˂ 0.05). CONCLUSION:RES addition may improve the biocompatibility without causing negative influence on μTBS of the adhesives. CLINICAL RELEVANCE: RES addition has clinical applicable potential to overcome the adverse biocompatibility of adhesives.
Entities:
Keywords:
Adhesive; Antioxidant; Cell viability; Microtensile bond strength; Resveratrol
Authors: Isabel Cristina Celerino de Moraes Porto; Arthur Bezerra de Barros Rocha; Iverson Iago Soares Ferreira; Bruna Muritiba de Barros; Eryck Canabarra Ávila; Matheus Corrêa da Silva; Marcos Paulo Santana de Oliveira; Teresa de Lisieux Guedes Ferreira Lôbo; José Marcos Dos Santos Oliveira; Ticiano Gomes do Nascimento; Jeniffer Mclaine Duarte de Freitas; Johnnatan Duarte de Freitas Journal: Heliyon Date: 2021-02-19