Laura L Needham1, Laura Smy2, Megan A Lee1, Theresa M Kunzler1, Jonathan R Genzen3. 1. ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, United States. 2. ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, United States; Department of Pathology, University of Utah, 500 Chipeta Way, Salt Lake City, UT 84108, United States. 3. ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, United States; Department of Pathology, University of Utah, 500 Chipeta Way, Salt Lake City, UT 84108, United States; ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, United States. Electronic address: jonathan.genzen@path.utah.edu.
Abstract
BACKGROUND: Lipoprotein subclass analysis by nuclear magnetic resonance (NMR) can be used in risk assessment of atherosclerotic cardiovascular disease (ASCVD). There is little information in the literature regarding phlebotomy tube interferences with NMR testing. METHODS: Pooled human serum was exposed to phlebotomy tubes manufactured by Becton Dickinson (BD), Greiner Bio-One, or Sarstedt. Serum was analyzed on the Axinon lipoFIT by NMR assay and by conventional lipid assays performed on a Roche Cobas 8000 system. The effect of incomplete fill volume was also assessed. RESULTS: Analytical interference in NMR lipoprotein subclass results was observed across many different tube types. The 5 mL Greiner Bio-One Z Serum Sep Clot Activator tube correlated the best with non-gel containing serum tubes from BD and Greiner Bio-One. BD Serum Separator Tubes (SSTs) displayed strong interferences across several NMR analytes that were enhanced with decreased tube fill volumes. Interferences were also observed with different sizes of Greiner Bio-One Z Serum Sep Clot Activator tubes. Interference was generally not observed with conventional lipid testing, although minor interference was found for some tubes with lipoprotein(a) [Lp(a)]. CONCLUSIONS: NMR lipoprotein subclass analysis should be standardized by both tube type and tube size to prevent risk of analytical interference.
BACKGROUND: Lipoprotein subclass analysis by nuclear magnetic resonance (NMR) can be used in risk assessment of atherosclerotic cardiovascular disease (ASCVD). There is little information in the literature regarding phlebotomy tube interferences with NMR testing. METHODS: Pooled human serum was exposed to phlebotomy tubes manufactured by Becton Dickinson (BD), Greiner Bio-One, or Sarstedt. Serum was analyzed on the Axinon lipoFIT by NMR assay and by conventional lipid assays performed on a Roche Cobas 8000 system. The effect of incomplete fill volume was also assessed. RESULTS: Analytical interference in NMR lipoprotein subclass results was observed across many different tube types. The 5 mL Greiner Bio-One Z Serum Sep Clot Activator tube correlated the best with non-gel containing serum tubes from BD and Greiner Bio-One. BD Serum Separator Tubes (SSTs) displayed strong interferences across several NMR analytes that were enhanced with decreased tube fill volumes. Interferences were also observed with different sizes of Greiner Bio-One Z Serum Sep Clot Activator tubes. Interference was generally not observed with conventional lipid testing, although minor interference was found for some tubes with lipoprotein(a) [Lp(a)]. CONCLUSIONS: NMR lipoprotein subclass analysis should be standardized by both tube type and tube size to prevent risk of analytical interference.
Authors: Martin Rief; Reinhard Raggam; Peter Rief; Philipp Metnitz; Tatjana Stojakovic; Markus Reinthaler; Marianne Brodmann; Winfried März; Hubert Scharnagl; Günther Silbernagel Journal: Biomedicines Date: 2022-07-21
Authors: Thomas Metzner; Deborah R Leitner; Karin Mellitzer; Andrea Beck; Harald Sourij; Tatjana Stojakovic; Gernot Reishofer; Winfried März; Ulf Landmesser; Hubert Scharnagl; Hermann Toplak; Günther Silbernagel Journal: Biomedicines Date: 2022-01-17