Sergio Ortiz1, Marylin Lecsö-Bornet2, Christine Bonnal3, Sandrine Houze4, Sylvie Michel5, Raphaël Grougnet6, Sabrina Boutefnouchet7. 1. Laboratoire de Pharmacognosie CNRS-UMR 8638, Université Paris Descartes Sorbonne Paris Cité, Faculté Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France. Electronic address: sergio.ortiz.aguirre@gmail.com. 2. Laboratoire Ecosystème Intestinal, Probiotiques, Antibiotiques-EA 4065, Université Paris Descartes Sorbonne Paris Cité, Faculté Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, Paris, France. Electronic address: marylin.lecso@parisdescartes.fr. 3. Laboratoire de Parasitologie-Mycologie, Hôpital Bichat Claude Bernard, 46 rue Henri Huchard, Paris 75018, France. Electronic address: christine.bonnal@aphp.fr. 4. Laboratoire de Parasitologie-Mycologie, Hôpital Bichat Claude Bernard, 46 rue Henri Huchard, Paris 75018, France. Electronic address: sandrine.houze@bch.aphp.fr. 5. Laboratoire de Pharmacognosie CNRS-UMR 8638, Université Paris Descartes Sorbonne Paris Cité, Faculté Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France. Electronic address: sylvie.michel@parisdescartes.fr. 6. Laboratoire de Pharmacognosie CNRS-UMR 8638, Université Paris Descartes Sorbonne Paris Cité, Faculté Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France. Electronic address: raphael.grougnet@parisdescartes.fr. 7. Laboratoire de Pharmacognosie CNRS-UMR 8638, Université Paris Descartes Sorbonne Paris Cité, Faculté Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France. Electronic address: sabrina.boutefnouchet@parisdescartes.fr.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Previous ethnobotanical surveys from the north Andean part of Chile, where different ethnic groups are co-existing, with the preeminence of Aymara and Atacama traditions, revealed an extensive domestic use of the local flora. In these communities, traditional medicinal uses are mainly related to the treatment of respiratory, gastro-intestinal and urinary disorders, pain and inflammation, which is closely linked to epidemiological observations. AIM OF THE STUDY: As these symptoms may be related to infectious diseases, a bioguided evaluation of antibacterial and antifungal activity was conducted on eighteen species selected with the Taira community, in Ollague. MATERIALS AND METHODS: Screening was performed using a large panel of pathogenic germs involved in the main community acquired infectious diseases, represented by Gram positive and Gram negative bacteria of clinical interest and by human pathogenic fungi, using a bioguided approach. RESULTS AND CONCLUSIONS: Gram positive strains of clinical interest were highly sensitive to Aloysia deserticola (Verbenaceae) and Krameria lappacea (Krameriaceae) extracts. The bioguided approach led us to identify the isolated neolignan from K. lappacea conocarpan (1), and triterpenoids form A. deserticola (oleanolic acid (6) and ursolic acid (10)), as the main bioactive compounds.
ETHNOPHARMACOLOGICAL RELEVANCE: Previous ethnobotanical surveys from the north Andean part of Chile, where different ethnic groups are co-existing, with the preeminence of Aymara and Atacama traditions, revealed an extensive domestic use of the local flora. In these communities, traditional medicinal uses are mainly related to the treatment of respiratory, gastro-intestinal and urinary disorders, pain and inflammation, which is closely linked to epidemiological observations. AIM OF THE STUDY: As these symptoms may be related to infectious diseases, a bioguided evaluation of antibacterial and antifungal activity was conducted on eighteen species selected with the Taira community, in Ollague. MATERIALS AND METHODS: Screening was performed using a large panel of pathogenic germs involved in the main community acquired infectious diseases, represented by Gram positive and Gram negative bacteria of clinical interest and by human pathogenic fungi, using a bioguided approach. RESULTS AND CONCLUSIONS: Gram positive strains of clinical interest were highly sensitive to Aloysia deserticola (Verbenaceae) and Krameria lappacea (Krameriaceae) extracts. The bioguided approach led us to identify the isolated neolignan from K. lappacea conocarpan (1), and triterpenoids form A. deserticola (oleanolic acid (6) and ursolic acid (10)), as the main bioactive compounds.
Authors: Carlo Genovese; Floriana D'Angeli; Francesco Bellia; Alfio Distefano; Mariarita Spampinato; Francesco Attanasio; Daria Nicolosi; Valentina Di Salvatore; Gianna Tempera; Debora Lo Furno; Giuliana Mannino; Fabio Milardo; Giovanni Li Volti Journal: Antibiotics (Basel) Date: 2021-04-13