| Literature DB >> 30412369 |
Karen L Elbing1, Roger Brent2.
Abstract
We describe the procedure for inoculating overnight (starter) cultures of E. coli from a single colony, along with considerations for growing larger cultures. We also include two methods for monitoring the number of cells per unit volume (density) of liquid cultures using a spectrophotometer and a hemacytometer or "count slide."Entities:
Keywords: Escherichia coli K-12; aeration; cell counting; hemacytometer; spectrophotometer
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Year: 2018 PMID： 30412369 PMCID： PMC6375084 DOI： 10.1002/cpmb.81
Source DB: PubMed Journal: Curr Protoc Mol Biol ISSN： 1934-3647