Mohsen Saleh ElAlfy1, Fatma Soliman Elsayed Ebeid2, Tarek Mostafa Kamal3, Deena Samir Eissa4, Eman Abdel Rahman Ismail5, Samar Hassan Mohamed6. 1. Pediatrics Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: elalfyms@hotmail.com. 2. Pediatrics Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: dr.fatma_ebeid@yahoo.com. 3. Medical Genetics Pediatric Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: kamaltmgen@yahoo.com. 4. Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: dr.deena_samir@yahoo.com. 5. Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: eman.ismail_70@yahoo.com. 6. Pediatrics Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: samarmohamedagt@yahoo.com.
Abstract
BACKGROUND: Cerebrovascular stroke is a common critical complication of sickle cell disease (SCD). Angiotensinogen (AGT) M235T gene polymorphism is associated with risk of ischemic stroke and cardiovascular disease. AIM: We investigated the potential association between angiotensinogen M235T gene polymorphism and susceptibility to cerebrovascular and cardiopulmonary complications in adolescents with SCD. METHODS: Forty-six patients with SCD in steady state were studied stressing on history of stroke, hydroxyurea/chelation therapy, hematological profile, and echocardiographic findings. Polymerase chain reaction-based restriction fragment length polymorphism analysis was used to detect AGT M235T gene polymorphism. Fifty sex- and age-matched healthy controls were enrolled for assessment of M235T gene polymorphism pattern. RESULTS: The distribution of AGT M235T gene polymorphism was similar between SCD patients and healthy controls. The frequency of T allele of AGT M235T gene polymorphism (TT and MT genotypes) was significantly higher among patients with history of manifest stroke (P < .001). Patients with TT and MT genotypes had higher incidence of cardiopulmonary complications (P = .041) as well as higher percentage of HbS (P < .001) and lower hemoglobin level (P = .008) compared with those with MM genotype. Serum ferritin, liver iron concentration, and cardiac T2* were not related to T alleles or genotypes. Logistic regression analysis revealed that M235T genotype was a significant independent factor related to the occurrence of stroke among patients with SCD (Odds Ratio 14.05, 95% confidence interval 3.82-28.91; P = .001). CONCLUSION: AGT M235T gene polymorphism may represent a genetic modifier to vascular morbidities in Egyptian patients with SCD.
BACKGROUND:Cerebrovascular stroke is a common critical complication of sickle cell disease (SCD). Angiotensinogen (AGT) M235T gene polymorphism is associated with risk of ischemic stroke and cardiovascular disease. AIM: We investigated the potential association between angiotensinogenM235T gene polymorphism and susceptibility to cerebrovascular and cardiopulmonary complications in adolescents with SCD. METHODS: Forty-six patients with SCD in steady state were studied stressing on history of stroke, hydroxyurea/chelation therapy, hematological profile, and echocardiographic findings. Polymerase chain reaction-based restriction fragment length polymorphism analysis was used to detect AGTM235T gene polymorphism. Fifty sex- and age-matched healthy controls were enrolled for assessment of M235T gene polymorphism pattern. RESULTS: The distribution of AGTM235T gene polymorphism was similar between SCDpatients and healthy controls. The frequency of T allele of AGTM235T gene polymorphism (TT and MT genotypes) was significantly higher among patients with history of manifest stroke (P < .001). Patients with TT and MT genotypes had higher incidence of cardiopulmonary complications (P = .041) as well as higher percentage of HbS (P < .001) and lower hemoglobin level (P = .008) compared with those with MM genotype. Serum ferritin, liver iron concentration, and cardiac T2* were not related to T alleles or genotypes. Logistic regression analysis revealed that M235T genotype was a significant independent factor related to the occurrence of stroke among patients with SCD (Odds Ratio 14.05, 95% confidence interval 3.82-28.91; P = .001). CONCLUSION:AGTM235T gene polymorphism may represent a genetic modifier to vascular morbidities in Egyptian patients with SCD.
Authors: Rajesh N Kalaria; Mayowa O Owolabi; Rufus O Akinyemi; Bruce Ovbiagele; Olaleye A Adeniji; Fred S Sarfo; Foad Abd-Allah; Thierry Adoukonou; Okechukwu S Ogah; Pamela Naidoo; Albertino Damasceno; Richard W Walker; Adesola Ogunniyi Journal: Nat Rev Neurol Date: 2021-09-15 Impact factor: 42.937