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Literature DB >> 3040811

Application of chromosomal restriction endonuclease digest analysis for use as typing method for Clostridium difficile.

Abstract

The usefulness of restriction endonuclease analysis of chromosomal DNA as a typing method for Clostridium difficile was tested. Over four months all faecal samples were routinely cultured for C difficile. DNA of all isolated strains was isolated and tested with the restriction endonuclease Hind III. The patterns obtained after electrophoresis in agarose gels seemed to be strain specific. Antibiotic susceptibility profiles agreed with the results of the restriction endonuclease analysis, though they were much less discriminating. Analysis of the results indicated that restriction endonuclease analysis is a suitable typing method for C difficile, which may be very valuable in epidemiological studies where a highly discriminating typing method is needed.

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Year: 1987 PMID： 3040811 PMCID： PMC1141096 DOI： 10.1136/jcp.40.7.771

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

15 in total

1. Cytotoxicity assay in antibiotic-associated colitis.

Authors: T W Chang; M Lauermann; J G Bartlett
Journal: J Infect Dis Date: 1979-11 Impact factor: 5.226

2. Clostridium difficile and its toxin in healthy neonates.

Authors: S A Richardson; P A Alcock; J Gray
Journal: Br Med J (Clin Res Ed) Date: 1983-09-24

3. In vitro susceptibility of Clostridium difficile isolates from patients with antibiotic-associated diarrhea or colitis.

Authors: J Dzink; J G Bartlett
Journal: Antimicrob Agents Chemother Date: 1980-04 Impact factor: 5.191

4. Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis.

Authors: W C Bradbury; A D Pearson; M A Marko; R V Congi; J L Penner
Journal: J Clin Microbiol Date: 1984-03 Impact factor: 5.948

5. Identification of Leptospira serovars by restriction-endonuclease analysis.

Authors: R B Marshall; B E Wilton; A J Robinson
Journal: J Med Microbiol Date: 1981-02 Impact factor: 2.472

6. Outbreaks of diarrhea associated with Clostridium difficile and its toxin in day-care centers: evidence of person-to-person spread.

Authors: K Kim; H L DuPont; L K Pickering
Journal: J Pediatr Date: 1983-03 Impact factor: 4.406

7. Identification of outbreak-associated and other strains of Clostridium difficile by numerical analysis of SDS-PAGE protein patterns.

Authors: M Costas; B Holmes; M Ganner; S L On; P N Hoffman; M A Worsley; H Panigrahi
Journal: Epidemiol Infect Date: 1994-08 Impact factor: 2.451

7 in total

Application of chromosomal restriction endonuclease digest analysis for use as typing method for Clostridium difficile.

1. Cytotoxicity assay in antibiotic-associated colitis.

2. Clostridium difficile and its toxin in healthy neonates.

3. In vitro susceptibility of Clostridium difficile isolates from patients with antibiotic-associated diarrhea or colitis.

4. Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis.

5. Identification of Leptospira serovars by restriction-endonuclease analysis.

6. Outbreaks of diarrhea associated with Clostridium difficile and its toxin in day-care centers: evidence of person-to-person spread.

7. Investigation of an outbreak of antibiotic-associated colitis by various typing methods.

8. Epidemiology of Clostridium difficile in infants.

9. Use of sodium taurocholate to enhance spore recovery on a medium selective for Clostridium difficile.

10. Isolation rates and toxigenic potential of Clostridium difficile isolates from various patient populations.

1. Characterization of flagella of Clostridium difficile and their role in serogrouping reactions.

2. Use of arbitrary primer PCR to type Clostridium difficile and comparison of results with those by immunoblot typing.

3. Typing of Clostridium difficile causing diarrhoea in an orthopaedic ward.

4. A molecular characterization of Clostridium difficile isolates from humans, animals and their environments.

5. Relapse versus reinfection with Clostridium difficile.

Review 6. Epidemiological aspects of infections caused by Bacteroides fragilis and Clostridium difficile.

7. Identification of outbreak-associated and other strains of Clostridium difficile by numerical analysis of SDS-PAGE protein patterns.