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Literature DB >> 3040474

Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of a type-2A protein phosphatase.

O B da Cruz e Silva, S Alemany, D G Campbell, P T Cohen.

Abstract

A 2.5 kb clone containing the full-length coding sequence of a type-2A protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in lambda gt10. The sequence of the protein deduced from the cDNA contains 309 residues (35.58 kDa). A major mRNA species at 2.0 kb and a minor component at 2.8 kb were visualized by Northern blotting in both skeletal muscle and liver. The type-2A enzyme showed weak homology with mammalian alkaline phosphatases between residues 55 and 95. The protein sequence of the type-2A phosphatase from rabbit skeletal muscle differs from that reported for the bovine adrenal enzyme in three regions.

Entities: Gene Species

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Year: 1987 PMID： 3040474 DOI： 10.1016/0014-5793(87)80966-3

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

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Cited

23 in total

Review 1. The regulation and function of protein phosphatases in the brain.

Authors: A T Sim
Journal: Mol Neurobiol Date: 1991 Impact factor: 5.590

2. The Saccharomyces cerevisiae genes (CMP1 and CMP2) encoding calmodulin-binding proteins homologous to the catalytic subunit of mammalian protein phosphatase 2B.

Authors: Y Liu; S Ishii; M Tokai; H Tsutsumi; O Ohki; R Akada; K Tanaka; E Tsuchiya; S Fukui; T Miyakawa
Journal: Mol Gen Genet Date: 1991-05

10. Identification of binding sites on the regulatory A subunit of protein phosphatase 2A for the catalytic C subunit and for tumor antigens of simian virus 40 and polyomavirus.

Authors: R Ruediger; D Roeckel; J Fait; A Bergqvist; G Magnusson; G Walter
Journal: Mol Cell Biol Date: 1992-11 Impact factor: 4.272

Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of a type-2A protein phosphatase.

Review 1. The regulation and function of protein phosphatases in the brain.

2. The Saccharomyces cerevisiae genes (CMP1 and CMP2) encoding calmodulin-binding proteins homologous to the catalytic subunit of mammalian protein phosphatase 2B.

3. The SIT4 protein phosphatase functions in late G1 for progression into S phase.

4. Autophosphorylation-activated protein kinase phosphorylates and inactivates protein phosphatase 2A.

5. Association of protein phosphatase 2A with polyoma virus medium tumor antigen.

6. Cloning of human calcineurin A: evidence for two isozymes and identification of a polyproline structural domain.

7. Multiple genes encoding serine/threonine protein phosphatases and their differential expression in Nicotiana tabacum.

8. Expression of protein phosphatases during postnatal development of rabbit heart.

9. Genetic interaction between the Ras-cAMP pathway and the Dis2s1/Glc7 protein phosphatase in Saccharomyces cerevisiae.

10. Identification of binding sites on the regulatory A subunit of protein phosphatase 2A for the catalytic C subunit and for tumor antigens of simian virus 40 and polyomavirus.