[Molecular cloning of genes coding for tumor necrosis factor. Complete nucleotide sequence of the genome copy of TNF-alpha in mice].

Using Sanger's technique and synthetic oligonucleotides derived from the known structure of cDNA, complete nucleotide sequence of the genomic copy of the mouse TNF-alpha gene has been determined. The length of the gene from transcription initiation site down to polyadenylation signal is about 2620 bp. Comparison with the previously described cDNA sequence revealed existence of 4 exons and 3 introns in the positions homologous to those of human TNF gene. The fourth exon codes for 88% amino acids of mature TNF-alpha.