Nancy S Saad1, Mohammad T Elnakish1, Elizabeth A Brundage2, Brandon J Biesiadecki2, Ahmet Kilic3, Amany A E Ahmed4, Peter J Mohler2, Paul M L Janssen5. 1. Department of Physiology and Cell Biology, College of Medicine, The Ohio State University, Columbus, OH, USA; Dorothy M. Davis Heart & Lung Research Institute, The Ohio State University, Columbus, OH, USA; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Helwan University, Cairo, Egypt. 2. Department of Physiology and Cell Biology, College of Medicine, The Ohio State University, Columbus, OH, USA; Dorothy M. Davis Heart & Lung Research Institute, The Ohio State University, Columbus, OH, USA. 3. Dorothy M. Davis Heart & Lung Research Institute, The Ohio State University, Columbus, OH, USA; Department of Surgery, College of Medicine, The Ohio State University, Columbus, OH, USA. 4. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Helwan University, Cairo, Egypt. 5. Department of Physiology and Cell Biology, College of Medicine, The Ohio State University, Columbus, OH, USA; Dorothy M. Davis Heart & Lung Research Institute, The Ohio State University, Columbus, OH, USA. Electronic address: janssen.10@osu.edu.
Abstract
AIMS: Heart failure (HF) is a prevalent disease that is considered the foremost reason for hospitalization in the United States. Most protein kinases (PK) are activated in heart disease and their inhibition has been shown to improve cardiac function in both animal and human studies. However, little is known about the direct impact of PKA and PKC inhibitors on human cardiac contractile function. MATERIAL AND METHODS: We investigated the ex vivo effect of such inhibitors on force as well as on kinetics of left ventricular (LV) trabeculae dissected from non-failing and failing human hearts. In these experiments, we applied 0.5 μM of H-89 and GF109203X, which are PKA and PKC inhibitors, respectively, in comparison to their vehicle DMSO (0.05%). KEY FINDINGS AND CONCLUSION: Statistical analyses revealed no significant effect for H-89 and GF109203X on either contractile force or kinetics parameters of both non-failing and failing muscles even though they were used at a concentration higher than the reported IC50s and Kis. Therefore, several factors such as selectivity, concentration, and treatment time, which are related to these PK inhibitors according to previous studies require further exploration.
AIMS: Heart failure (HF) is a prevalent disease that is considered the foremost reason for hospitalization in the United States. Most protein kinases (PK) are activated in heart disease and their inhibition has been shown to improve cardiac function in both animal and human studies. However, little is known about the direct impact of PKA and PKC inhibitors on human cardiac contractile function. MATERIAL AND METHODS: We investigated the ex vivo effect of such inhibitors on force as well as on kinetics of left ventricular (LV) trabeculae dissected from non-failing and failing human hearts. In these experiments, we applied 0.5 μM of H-89 and GF109203X, which are PKA and PKC inhibitors, respectively, in comparison to their vehicle DMSO (0.05%). KEY FINDINGS AND CONCLUSION: Statistical analyses revealed no significant effect for H-89 and GF109203X on either contractile force or kinetics parameters of both non-failing and failing muscles even though they were used at a concentration higher than the reported IC50s and Kis. Therefore, several factors such as selectivity, concentration, and treatment time, which are related to these PK inhibitors according to previous studies require further exploration.
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Authors: Mohammed A Mashali; Nancy S Saad; Benjamin D Canan; Mohammad T Elnakish; Nima Milani-Nejad; Jae-Hoon Chung; Eric J Schultz; Salome A Kiduko; Amanda W Huang; Austin N Hare; Kyra K Peczkowski; Farbod Fazlollahi; Brit L Martin; Jason D Murray; Courtney M Campbell; Ahmet Kilic; Bryan A Whitson; Nahush A Mokadam; Peter J Mohler; Paul M L Janssen Journal: J Mol Cell Cardiol Date: 2021-03-22 Impact factor: 5.763
Authors: Nancy S Saad; Mohammed A Mashali; Mohammad T Elnakish; Austin Hare; Courtney M Campbell; Salome A Kiduko; Kyra K Peczkowski; Amanda W Huang; Farbod Fazlollahi; Gina S Torres Matias; Amany A E Ahmed; Bryan A Whitson; Nahush A Mokadam; Paul M L Janssen Journal: PLoS One Date: 2022-04-11 Impact factor: 3.240