Joana DE Fátima Ferreira Borges DA Costa1, Carla DE Castro Sant' Anna2,3, José Augusto Pereira Carneiro Muniz4, Carlos Alberto Machado DA Rocha5, Letícia Martins Lamarão6, Caroline DE Fátima Aquino Moreira Nunes1,7, Paulo Pimentel DE Assumpção3, Rommel Rodriguez Burbano1,8. 1. Laboratory of Human Cytogenetics, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil. 2. Laboratory of Molecular Biology, Ophir Loyola Hospital, Belém, Brazil santannacarla@yahoo.com.br. 3. Nucleus of Oncology Research, Federal University of Pará, Belém, Brazil. 4. National Primate Center, Ministry of Health, Ananindeua, Brazil. 5. Department of Teaching, Natural Resources, Design and Infrastructure, Federal Institute of Education, Science and Technology of Pará, Belém, Brazil. 6. Laboratory of Nucleic Acid Tests, Hematology and Hematology Center of Pará Foundation, Belém, Brazil. 7. Laboratory of Genetics of Hemoglobinopathies and Hematologic Diseases, Federal University of Ceará, Fortaleza, Brazil. 8. Laboratory of Molecular Biology, Ophir Loyola Hospital, Belém, Brazil.
Abstract
BACKGROUND/AIM: The evolution of gastric carcinogenesis remains largely unknown, as the regulatory mechanisms involved in the aggressiveness of gastric cancer are still poorly understood. Kinases are downstream modulators and effectors of various cell signaling cascades and play key roles in the development of neoplastic diseases. The objective of this study was to evaluate the expression of genes and proteins of the SRC family, including FYN, YES, BLK, FGR, LYN and SRC, in a model of intestinal gastric carcinogenesis generated by treating Cebus apella, a New World non-human primate, with N-methyl nitrosourea (MNU). MATERIALS AND METHODS: mRNA expression of genes was measured by real-time reverse transcription quantitative PCR (RT-qPCR) and protein expression was measured by western blotting in six Cebus apella treated with N-methyl-nitrosourea (MNU) for about 2.5 years. RESULTS: Elevated mRNA and protein expression mainly of the SRC and LYN kinases was observed. Their expression was gradually increasing as non-atrophic gastritis was evolving to gastric cancer. CONCLUSION: SRC family kinases play a key role in tumor progression and metastasis and may be a promising target for the treatment of gastric cancer. Copyright
BACKGROUND/AIM: The evolution of gastric carcinogenesis remains largely unknown, as the regulatory mechanisms involved in the aggressiveness of gastric cancer are still poorly understood. Kinases are downstream modulators and effectors of various cell signaling cascades and play key roles in the development of neoplastic diseases. The objective of this study was to evaluate the expression of genes and proteins of the SRC family, including FYN, YES, BLK, FGR, LYN and SRC, in a model of intestinal gastric carcinogenesis generated by treating Cebus apella, a New World non-human primate, with N-methyl nitrosourea (MNU). MATERIALS AND METHODS: mRNA expression of genes was measured by real-time reverse transcription quantitative PCR (RT-qPCR) and protein expression was measured by western blotting in six Cebus apella treated with N-methyl-nitrosourea (MNU) for about 2.5 years. RESULTS: Elevated mRNA and protein expression mainly of the SRC and LYN kinases was observed. Their expression was gradually increasing as non-atrophic gastritis was evolving to gastric cancer. CONCLUSION:SRC family kinases play a key role in tumor progression and metastasis and may be a promising target for the treatment of gastric cancer. Copyright
Authors: Mohd Altaf Najar; Mohammad Arefian; David Sidransky; Harsha Gowda; T S Keshava Prasad; Prashant Kumar Modi; Aditi Chatterjee Journal: Front Genet Date: 2022-05-13 Impact factor: 4.772