Kao-Hui Liu1,2, Yi-Ta Tsai2, Szu-Ying Chin1, Woan-Ruoh Lee1,2,3, Yen-Chou Chen2,4,5, Shing-Chuan Shen6,3,4. 1. Department of Dermatology, Taipei Medical University Shuang Ho Hospital, New Taipei City, Taiwan, R.O.C. 2. Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C. 3. Department of Dermatology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C. 4. International Master/PhD Program in Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C. 5. Cancer Research Center and Orthopedics Research Center, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C. 6. Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C. scshen@tmu.edu.tw.
Abstract
BACKGROUND/AIM: Recent studies implied a significant role of hypoxia-inducible factor-1α (HIF1α) in cell transformation. This study aimed to assess the effects of HIF1α on the epithelial-to-mesenchymal transition (EMT) and tumorigenesis of lung adenocarcinoma cells. MATERIALS AND METHODS: Invasion, migration and colony formation assays were used to evaluate cell transformation. Expression of EMT-related markers were analyzed by western blot, reverse-transcription polymerase chain reaction or zymography. A luciferase assay was carried out to access the transcriptional activity of β-catenin. RESULTS: Hypoxia enhanced migration, invasion and transformation of A549 lung adenocarcinoma cells. Hypoxic stimulation promoted the expression of EMT-related markers in lung cancer cells. The expression of HIF1α was found to be involved in hypoxia-mediated modulation of expression of snail family transcriptional repressors 1 (SNAI1) and 2 (SLUG). Hypoxia enhanced nuclear accumulation and transcriptional activity of β-catenin. CONCLUSION: β-Catenin promotes expression of EMT-related genes and eventually contributes to the metastatic process. Copyright
BACKGROUND/AIM: Recent studies implied a significant role of hypoxia-inducible factor-1α (HIF1α) in cell transformation. This study aimed to assess the effects of HIF1α on the epithelial-to-mesenchymal transition (EMT) and tumorigenesis of lung adenocarcinoma cells. MATERIALS AND METHODS: Invasion, migration and colony formation assays were used to evaluate cell transformation. Expression of EMT-related markers were analyzed by western blot, reverse-transcription polymerase chain reaction or zymography. A luciferase assay was carried out to access the transcriptional activity of β-catenin. RESULTS:Hypoxia enhanced migration, invasion and transformation of A549 lung adenocarcinoma cells. Hypoxic stimulation promoted the expression of EMT-related markers in lung cancer cells. The expression of HIF1α was found to be involved in hypoxia-mediated modulation of expression of snail family transcriptional repressors 1 (SNAI1) and 2 (SLUG). Hypoxia enhanced nuclear accumulation and transcriptional activity of β-catenin. CONCLUSION: β-Catenin promotes expression of EMT-related genes and eventually contributes to the metastatic process. Copyright
Authors: Laura Saieva; Maria Magdalena Barreca; Chiara Zichittella; Maria Giulia Prado; Marco Tripodi; Riccardo Alessandro; Alice Conigliaro Journal: Int J Mol Sci Date: 2020-05-28 Impact factor: 5.923
Authors: Juha M T Hyttinen; Ram Kannan; Szabolcs Felszeghy; Minna Niittykoski; Antero Salminen; Kai Kaarniranta Journal: Int J Mol Sci Date: 2019-11-18 Impact factor: 5.923