Defragmentation of lysozyme derived Amyloid β fibril using Biocompatible Magnetic fluid.

We present here a modulating effect on lysozyme derived Amyloid β fibrils by aqueous magnetic fluid. This non-conventional approach of treatment of lysozyme derived Amyloid β fibrils showed lysing of Amyloid fibrils to its secondary structures which can be seen using optical microscope and scanning electron microscopic image. The size of lysozyme derived amyloid fibrils before and after treatment was measured using dynamic light scattering technique. The mechanism of defragmentation of lysozyme derived Amyloid β fibrils by magnetic fluid is explained. This is a first report to identify the secondary structure of protein using Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) spectra after lysing. The cyto-toxicity study of this magnetic fluid on neuronal (SH-SY5Y) and non-neuronal (NRK) cell lines shows non-toxicity up to a concentration of 250 μg/mL. The study indicates a novel and unique complementary approach to treat the amyloidogenic brain diseases.