D Loncaric1, V Labat1, C Debeissat1, P Brunet de la Grange1, L Rodriguez1, M Vlaski-Lafarge1, Z Ivanovic2. 1. Établissement français du sang Nouvelle Aquitaine, scientific department, place Amélie-Raba-Léon, CS21010, 33075 Bordeaux cedex, France; Inserm U1035, université de Bordeaux, 33000 Bordeaux, France. 2. Établissement français du sang Nouvelle Aquitaine, scientific department, place Amélie-Raba-Léon, CS21010, 33075 Bordeaux cedex, France; Inserm U1035, université de Bordeaux, 33000 Bordeaux, France. Electronic address: zoran.ivanovc@efs.sante.fr.
Abstract
OBJECTIVES: The first-passage adherent human bone marrow fibroblast-like cell population corresponds, in terms of phenotype and three-lineage differentiation capacity (assayed in bulk culture), to commonly termed "mesenchymal stem cells". Here we determine the proportion of high proliferative capacity multipotent cells present in this population in order to estimate the proportion of cells that can or cannot be considered as stem and progenitor cells. MATERIAL AND METHODS: The single-cell cultures were established starting from human bone marrow-derived first-passage fibroblast-like cells and the proliferating clones were either transferred to secondary cultures to evaluate their further clonogenicity, or split into three wells to assess differentiation into each of the three different lineages. RESULTS: The analysis of 197 single-cell cultures from three different bone marrow donors shows that only∼40% of so-called "mesenchymal stem cells" exhibit multipotency and are capable of sustained clonogenicity in secondary cultures. CONCLUSION: Even in the first ex vivo passage under favorable conditions the majority (∼60%) of so-called "mesenchymal stem cells" are not multipotent and thus do not represent a stem cell entity.
OBJECTIVES: The first-passage adherent human bone marrow fibroblast-like cell population corresponds, in terms of phenotype and three-lineage differentiation capacity (assayed in bulk culture), to commonly termed "mesenchymal stem cells". Here we determine the proportion of high proliferative capacity multipotent cells present in this population in order to estimate the proportion of cells that can or cannot be considered as stem and progenitor cells. MATERIAL AND METHODS: The single-cell cultures were established starting from human bone marrow-derived first-passage fibroblast-like cells and the proliferating clones were either transferred to secondary cultures to evaluate their further clonogenicity, or split into three wells to assess differentiation into each of the three different lineages. RESULTS: The analysis of 197 single-cell cultures from three different bone marrow donors shows that only∼40% of so-called "mesenchymal stem cells" exhibit multipotency and are capable of sustained clonogenicity in secondary cultures. CONCLUSION: Even in the first ex vivo passage under favorable conditions the majority (∼60%) of so-called "mesenchymal stem cells" are not multipotent and thus do not represent a stem cell entity.
Authors: Marija Vlaski-Lafarge; Veronique Labat; Alexandra Brandy; Alice Refeyton; Pascale Duchez; Laura Rodriguez; Nyere Gibson; Philippe Brunet de la Grange; Zoran Ivanovic Journal: Front Oncol Date: 2020-05-12 Impact factor: 6.244