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Literature DB >> 30381480

High-Throughput Single-Cell Sequencing of both TCR-β Alleles.

Tomonori Hosoya¹, Hongyang Li², Chia-Jui Ku¹, Qingqing Wu¹, Yuanfang Guan², James Douglas Engel³.

Abstract

Allelic exclusion is a vital mechanism for the generation of monospecificity to foreign Ags in B and T lymphocytes. In this study, we developed a high-throughput barcoded method to simultaneously analyze the VDJ recombination status of both mouse TCR-β alleles in hundreds of single cells using next-generation sequencing.

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Year: 2018 PMID： 30381480 DOI： 10.4049/jimmunol.1800774

Source DB: PubMed Journal: J Immunol ISSN： 0022-1767 Impact factor: 5.422

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Cited

4 in total

1. Micro-dissection and integration of long and short reads to create a robust catalog of kidney compartment-specific isoforms.

Authors: Hongyang Li; Ridvan Eksi; Daiyao Yi; Bradley Godfrey; Lisa R Mathew; Christopher L O'Connor; Markus Bitzer; Matthias Kretzler; Rajasree Menon; Yuanfang Guan
Journal: PLoS Comput Biol Date: 2022-04-25 Impact factor: 4.475

High-Throughput Single-Cell Sequencing of both TCR-β Alleles.

1. Micro-dissection and integration of long and short reads to create a robust catalog of kidney compartment-specific isoforms.

2. Clonotype pattern in T-cell lymphomas map the cell of origin to immature lymphoid precursors.

Review 3. Machine Learning Approaches to TCR Repertoire Analysis.

Review 4. Specificity of the T Cell Response to Protein Biopharmaceuticals.