| Literature DB >> 30377379 |
Hong Qiu1,2, Songshan Shi1,3, Jingwen Yue1,2, Meng Xin1,2, Alison V Nairn1, Lei Lin4, Xinyue Liu4, Guoyun Li5, Stephanie A Archer-Hartmann1, Mitche Dela Rosa1, Melina Galizzi1, Shunchun Wang1,3, Fuming Zhang4, Parastoo Azadi1, Toin H van Kuppevelt6, Wellington V Cardoso7, Koji Kimata8, Xingbin Ai9, Kelley W Moremen1,2, Jeffrey D Esko10, Robert J Linhardt4, Lianchun Wang11,12.
Abstract
Heparan sulfate (HS) is a complex linear polysaccharide that modulates a wide range of biological functions. Elucidating the structure-function relationship of HS has been challenging. Here we report the generation of an HS-mutant mouse lung endothelial cell library by systematic deletion of HS genes expressed in the cell. We used this library to (1) determine that the strictly defined fine structure of HS, not its overall degree of sulfation, is more important for FGF2-FGFR1 signaling; (2) define the epitope features of commonly used anti-HS phage display antibodies; and (3) delineate the fine inter-regulation networks by which HS genes modify HS and chain length in mammalian cells at a cell-type-specific level. Our mutant-cell library will allow robust and systematic interrogation of the roles and related structures of HS in a cellular context.Entities:
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Year: 2018 PMID： 30377379 PMCID： PMC6214364 DOI： 10.1038/s41592-018-0189-6
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547