Literature DB >> 30377371

A high-throughput pipeline for validation of antibodies.

Krzysztof Sikorski1,2, Adi Mehta1,3,4, Marit Inngjerdingen1, Flourina Thakor3,4, Simon Kling5, Tomas Kalina6, Tuula A Nyman1, Maria Ekman Stensland1, Wei Zhou7, Gustavo A de Souza8, Lars Holden9, Jan Stuchly6, Markus Templin5, Fridtjof Lund-Johansen10,11.   

Abstract

Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).

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Year:  2018        PMID： 30377371     DOI： 10.1038/s41592-018-0179-8

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  13 in total

1.  Using proteins to test for COVID antibodies.

Authors:  Helen Santoro
Journal:  Nature       Date:  2022-10       Impact factor: 69.504

2.  Immune responses in Omicron SARS-CoV-2 breakthrough infection in vaccinated adults.

Authors:  Ludvig A Munthe; Siri Mjaaland; Hassen Kared; Asia-Sophia Wolf; Amin Alirezaylavasani; Anthony Ravussin; Guri Solum; Trung The Tran; Fridtjof Lund-Johansen; John Torgils Vaage; Lise Sofie Nissen-Meyer; Unni C Nygaard; Olav Hungnes; Anna H Robertson; Lisbeth Meyer Næss; Lill Trogstad; Per Magnus
Journal:  Nat Commun       Date:  2022-07-18       Impact factor: 17.694

3.  High-resolution antibody array analysis of proteins from primary human keratinocytes and leukocytes.

Authors:  Daniel de la Rosa Carrillo; Krzysztof Sikorski; Denis Khnykin; Weiwei Wu; Fridtjof Lund-Johansen
Journal:  PLoS One       Date:  2018-12-27       Impact factor: 3.240

4.  Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.

Authors:  Carl Laflamme; Paul M McKeever; Rahul Kumar; Julie Schwartz; Mahshad Kolahdouzan; Carol X Chen; Zhipeng You; Faiza Benaliouad; Opher Gileadi; Heidi M McBride; Thomas M Durcan; Aled M Edwards; Luke M Healy; Janice Robertson; Peter S McPherson
Journal:  Elife       Date:  2019-10-15       Impact factor: 8.140

5.  Resistance training increases muscle NAD+ and NADH concentrations as well as NAMPT protein levels and global sirtuin activity in middle-aged, overweight, untrained individuals.

Authors:  Donald A Lamb; Johnathon H Moore; Paulo Henrique Caldeira Mesquita; Morgan A Smith; Christopher G Vann; Shelby C Osburn; Carlton D Fox; Hector L Lopez; Tim N Ziegenfuss; Kevin W Huggins; Michael D Goodlett; Andrew D Fruge; Andreas N Kavazis; Kaelin C Young; Michael D Roberts
Journal:  Aging (Albany NY)       Date:  2020-05-05       Impact factor: 5.682

6.  CD Maps-Dynamic Profiling of CD1-CD100 Surface Expression on Human Leukocyte and Lymphocyte Subsets.

Authors:  Tomas Kalina; Karel Fišer; Martin Pérez-Andrés; Daniela Kuzílková; Marta Cuenca; Sophinus J W Bartol; Elena Blanco; Pablo Engel; Menno C van Zelm
Journal:  Front Immunol       Date:  2019-10-23       Impact factor: 7.561

7.  Antibody validation for Western blot: By the user, for the user.

Authors:  Lakshmi Pillai-Kastoori; Sam Heaton; Steve D Shiflett; Annabelle C Roberts; Alejandra Solache; Amy R Schutz-Geschwender
Journal:  J Biol Chem       Date:  2019-12-09       Impact factor: 5.157

8.  Systemic complement activation is associated with respiratory failure in COVID-19 hospitalized patients.

Authors:  Jan C Holter; Soeren E Pischke; Eline de Boer; Andreas Lind; Synne Jenum; Aleksander R Holten; Kristian Tonby; Andreas Barratt-Due; Marina Sokolova; Camilla Schjalm; Viktoriia Chaban; Anette Kolderup; Trung Tran; Torleif Tollefsrud Gjølberg; Linda G Skeie; Liv Hesstvedt; Vidar Ormåsen; Børre Fevang; Cathrine Austad; Karl Erik Müller; Cathrine Fladeby; Mona Holberg-Petersen; Bente Halvorsen; Fredrik Müller; Pål Aukrust; Susanne Dudman; Thor Ueland; Jan Terje Andersen; Fridtjof Lund-Johansen; Lars Heggelund; Anne M Dyrhol-Riise; Tom E Mollnes
Journal:  Proc Natl Acad Sci U S A       Date:  2020-09-17       Impact factor: 11.205

9.  The Antibody Society's antibody validation webinar series.

Authors:  Jan L A Voskuil; Anita Bandrowski; C Glenn Begley; Andrew R M Bradbury; Andrew D Chalmers; Aldrin V Gomes; Travis Hardcastle; Fridtjof Lund-Johansen; Andreas Plückthun; Giovanna Roncador; Alejandra Solache; Michael J Taussig; James S Trimmer; Cecilia Williams; Simon L Goodman
Journal:  MAbs       Date:  2020 Jan-Dec       Impact factor: 5.857

10.  Deepening into Intracellular Signaling Landscape through Integrative Spatial Proteomics and Transcriptomics in a Lymphoma Model.

Authors:  Alicia Landeira-Viñuela; Paula Díez; Pablo Juanes-Velasco; Quentin Lécrevisse; Alberto Orfao; Javier De Las Rivas; Manuel Fuentes
Journal:  Biomolecules       Date:  2021-11-26
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