Ercan Dinçel1, Yeşim Özkan2, Murat Şüküroğlu3, Hakan Özsoy1, Aylin Sepici Dinçel4. 1. Department of Orthopedics and Traumatology, University of Health Sciences, Ankara Training and Research Hospital, Ankara, Turkey. 2. Department of Biochemistry, University of Gazi, Faculty of Pharmacy, Ankara, Turkey. 3. Department of Pharmaceutical Chemistry, University of Gazi, Faculty of Pharmacy, Ankara, Turkey. 4. Department of Medical Biochemistry, University of Gazi, Faculty of Medicine, Ankara, Turkey.
Abstract
OBJECTIVES: This study aims to evaluate tryptophan degradation and clarify whether altered levels of kynurenine and tryptophan (Kyn/Trp) ratio could be correlated to osteoporotic hip fractures via immune system. PATIENTS AND METHODS: The study included 60 patients with osteoporotic hip fracture (20 males, 40 females, mean age 76.6±6.9 years; range 59 to 95 years). Patients were divided into two as patients with collum femoris fractures (group 1; n=23) and intertrochanteric fractures (group 2; n=37). Fifteen healthy subjects without any fracture were selected as control group (group 3; 3 males, 12 females; mean age 69.7±8.4; range 60 to 86 years). All fractures were simple falls due to low energy trauma. Bone mineral density measurements were performed with Lunar dual energy X-ray absorptiometry. Kyn/Trp levels were measured by high performance liquid chromatography. Interleukin (IL)-6 and IL-1 beta levels were measured with solid-phase sandwich enzyme-linked immunosorbent assay. RESULTS: All bone mineral density values were in agreement for osteoporosis and there was no significant difference between the two groups. Higher Kyn/Trp ratios were observed in groups 1 and 2 compared to group 3. This difference was more significant in group 1 (p=0.0001) than that in group 2 (p=0.048). Also, group 1 had significantly higher Kyn/Trp ratio than group 2 (p=0.011). There were significantly higher IL-6 and lower IL-1 beta levels both in groups 1 and 2 compared to group 3 (p=0.0001). There was no significant difference between group 1 and group 2 in terms of IL-6 and IL-1 beta levels. There was positive correlation with Kyn/Trp ratio (r=0.581, p=0.004) in group 2. Also, significant correlation was detected between IL-6 and IL-1 beta levels in the same group (r=0.665, p=0.036). CONCLUSION: Both increased degradation of tryptophan and ratio of Kyn/Trp indicate the relationship of immune activation with bone healing.
OBJECTIVES: This study aims to evaluate tryptophan degradation and clarify whether altered levels of kynurenine and tryptophan (Kyn/Trp) ratio could be correlated to osteoporotic hip fractures via immune system. PATIENTS AND METHODS: The study included 60 patients with osteoporotic hip fracture (20 males, 40 females, mean age 76.6±6.9 years; range 59 to 95 years). Patients were divided into two as patients with collum femoris fractures (group 1; n=23) and intertrochanteric fractures (group 2; n=37). Fifteen healthy subjects without any fracture were selected as control group (group 3; 3 males, 12 females; mean age 69.7±8.4; range 60 to 86 years). All fractures were simple falls due to low energy trauma. Bone mineral density measurements were performed with Lunar dual energy X-ray absorptiometry. Kyn/Trp levels were measured by high performance liquid chromatography. Interleukin (IL)-6 and IL-1 beta levels were measured with solid-phase sandwich enzyme-linked immunosorbent assay. RESULTS: All bone mineral density values were in agreement for osteoporosis and there was no significant difference between the two groups. Higher Kyn/Trp ratios were observed in groups 1 and 2 compared to group 3. This difference was more significant in group 1 (p=0.0001) than that in group 2 (p=0.048). Also, group 1 had significantly higher Kyn/Trp ratio than group 2 (p=0.011). There were significantly higher IL-6 and lower IL-1 beta levels both in groups 1 and 2 compared to group 3 (p=0.0001). There was no significant difference between group 1 and group 2 in terms of IL-6 and IL-1 beta levels. There was positive correlation with Kyn/Trp ratio (r=0.581, p=0.004) in group 2. Also, significant correlation was detected between IL-6 and IL-1 beta levels in the same group (r=0.665, p=0.036). CONCLUSION: Both increased degradation of tryptophan and ratio of Kyn/Trp indicate the relationship of immune activation with bone healing.
Entities:
Keywords:
3-dioxygenase; kynurenine; osteoporosis; tryptophan; Bone regeneration; indoleamine 2
Authors: Kamil E Barbour; Robert Boudreau; Michelle E Danielson; Ada O Youk; Jean Wactawski-Wende; Nancy C Greep; Andrea Z LaCroix; Rebecca D Jackson; Robert B Wallace; Douglas C Bauer; Matthew A Allison; Jane A Cauley Journal: J Bone Miner Res Date: 2012-05 Impact factor: 6.741
Authors: Georg Schett; Stefan Kiechl; Siegfried Weger; Angelo Pederiva; Agnes Mayr; Manuele Petrangeli; Friedrich Oberhollenzer; Rolando Lorenzini; Kurt Redlich; Roland Axmann; Jochen Zwerina; Johann Willeit Journal: Arch Intern Med Date: 2006 Dec 11-25
Authors: Shinya Ishii; Jane A Cauley; Gail A Greendale; Carolyn J Crandall; Michelle E Danielson; Yasuyoshi Ouchi; Arun S Karlamangla Journal: J Bone Miner Res Date: 2013-07 Impact factor: 6.741
Authors: E M Apalset; C G Gjesdal; P M Ueland; J Øyen; K Meyer; Ø Midttun; G E Eide; G S Tell Journal: Osteoporos Int Date: 2014-05-10 Impact factor: 4.507
Authors: Ahmed M Elmansi; Khaled A Hussein; Sergio Mas Herrero; Sudharsan Periyasamy-Thandavan; Alexandra Aguilar-Pérez; Galina Kondrikova; Dmitry Kondrikov; Nada H Eisa; Jessica L Pierce; Helen Kaiser; Ke-Hong Ding; Aisha L Walker; Xue Jiang; Wendy B Bollag; Mohammed Elsalanty; Qing Zhong; Xing-Ming Shi; Yun Su; Maribeth Johnson; Monte Hunter; Charles Reitman; Brian F Volkman; Mark W Hamrick; Carlos M Isales; Sadanand Fulzele; Meghan E McGee-Lawrence; William D Hill Journal: Bone Rep Date: 2020-04-23