Ayca Tuzcu1, Rabia Aydogan Baykara2, Ahmet Omma3, Gunseli Karaca Acet2, Erdal Dogan4, Medine Cumhur Cure5, Sevinc Can Sandikci6, Erkan Cure7, Salim Neşelioğlu8, Ozcan Erel8. 1. Department of Biochemistry, Malatya Education and Research Hospital, Malatya, Turkey. 2. Department of Physical Medicine and Rehabilitation, Malatya Education and Research Hospital, Malatya, Turkey. 3. Division of Rheumatology, Department of Internal Medicine, Numune Education and Research Hospital, Ankara, Turkey. 4. Department of Physical Medicine and Rehabilitation, Malatya Park Private Hospital, Malatya, Turkey. 5. Department of Biochemistry, Istanbul Laboratory, Istanbul Turkey. 6. Division of Rheumatology, Department of Internal Medicine, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey. 7. Department of Internal Medicine, Camlica Erdem Hospital, Istanbul, Turkey. 8. Department of Clinical Biochemistry, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey.
Abstract
BACKGROUND: Oxidative stress may play an important role in rheumatoid arthritis (RA) etiopathogenesis. The thiol group is a very strong antioxidant. In this study, we aimed to investigate the presence of oxidative stress in patients with RA by evaluating thiol/disulfide homeostasis. MATERIAL AND METHODS: A total of 50 female RA patients and 50 healthy female controls were included in this study. Thiol and disulfide values were calculated utilizing novel methods. RESULTS: Native thiol (p < 0.001) and total thiol (p < 0.001) levels of RA patients were significantly lower compared to values in the control group. However, the disulfide (p < 0.001) levels of RA patients were strongly higher than in healthy individuals. A negative correlation was found between thiol and disease activity score-28 among the patients, whereas a positive correlation was found between disulfide and disease activity score-28 among the patients. CONCLUSION: We found that the thiol-disulfide rate deteriorated in RA patients, with the proportion of disulfide increasing. There is a strong correlation between the decrease in thiol levels, increase in disulfide levels and the disease activity scores.
BACKGROUND: Oxidative stress may play an important role in rheumatoid arthritis (RA) etiopathogenesis. The thiol group is a very strong antioxidant. In this study, we aimed to investigate the presence of oxidative stress in patients with RA by evaluating thiol/disulfide homeostasis. MATERIAL AND METHODS: A total of 50 female RApatients and 50 healthy female controls were included in this study. Thiol and disulfide values were calculated utilizing novel methods. RESULTS: Native thiol (p < 0.001) and total thiol (p < 0.001) levels of RApatients were significantly lower compared to values in the control group. However, the disulfide (p < 0.001) levels of RApatients were strongly higher than in healthy individuals. A negative correlation was found between thiol and disease activity score-28 among the patients, whereas a positive correlation was found between disulfide and disease activity score-28 among the patients. CONCLUSION: We found that the thiol-disulfide rate deteriorated in RApatients, with the proportion of disulfide increasing. There is a strong correlation between the decrease in thiol levels, increase in disulfide levels and the disease activity scores.