Changes of metabolites of acrylamide and glycidamide in acrylamide-exposed rats pretreated with blueberry anthocyanins extract.

Blueberry anthocyanins extract (BAE) is widely used natural antioxidant. The inhibitory effect of BAE (175 mg/kg bw/day) pretreatment on glycidamide (GA) metabolism following acrylamide (AA) (35 mg/kg bw/day) gavage for consecutive 7 days and 14 days in SD rat model were investigated in this work. AA, GA and its metabolites in urine, blood and 3 tissues were quantified using UPLC-MS/MS, respectively. The results showed pretreatment with BAE could significantly block the epoxidation of AA to GA. Compared with the control, BAE pretreatment significantly decreased the amount of GA and its mercapturic acid metabolite N-acetyl-S-(3-amino-2-hydroxy-3-oxopropyl)-cysteine (GAMA3) in rats urine (p < 0.05), and sharply decreased the accumulation of GA DNA adduct N7-(2-carbamoyl-2-hydroxyethyl) guanine (N7-GA-Gua) in liver, kidney and lung (p < 0.05). Meanwhile, significant reduction (p < 0.05) of N-(2-carbamoyl-2-hydroxyethyl) valine (GA-VAL) content was found in erythrocyte of the rats treated with BAE. This study provides a novel evidence for elucidating the chemopreventive effect of BAE on AA toxicity.