Mohammed Ziaur Rahman1, Mariya Sumiya1, Mohammed Sahabuddin1, Lisa G Pell2, Jonathan B Gubbay3,4, Rajibur Rahman1, Farhana Momtaz1, Nafisa Azmuda5, Shaila S Shanta6, Ishrat Jahan7, Mustafizur Rahman1, Abdullah A Mahmud6, Daniel E Roth2,3, Shaun K Morris2,3. 1. Infectious Diseases Division (IDD), icddr,b, Dhaka, Bangladesh. 2. Centre for Global Child Health and Child Health Evaluative Sciences, Sick Kids Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada. 3. Department of Pediatrics, Hospital for Sick Children and University of Toronto, Toronto, Ontario, Canada. 4. Public Health Ontario, Toronto, Ontario, Canada. 5. Department of Microbiology, Jahangirnagar University, Dhaka, Bangladesh. 6. Nutrition and Clinical Services Division (NCSD), icddr,b, Dhaka, Bangladesh. 7. Maternal and Child Health Training Institute (MCHTI), Ministry of Health and Family Welfare, Government of Bangladesh, Dhaka, Bangladesh.
Abstract
BACKGROUND: Acute respiratory infection (ARI) is a leading cause of morbidity and mortality in children in low and middle-income countries. Human metapneumovirus (hMPV) is one of the most common viral etiological agents for ARIs in children. OBJECTIVES: In this study, we explored the genotypic diversity and the epidemiology of hMPV among infants in Dhaka, Bangladesh. STUDY DESIGN: Between December 2014 and August 2016, a total of 3810 mid-turbinate nasal swab samples were collected from infants (0 to 6 months of age) who met clinical ARI criteria, as a part of a prospective ARI cohort study. hMPV was detected using polymerase chain reaction, and genotyped by sequencing and phylogenetic analysis. RESULTS: hMPV was identified in 206 (5.4%) nasal swab specimens. One-tenth of the hMPV-positive swabs (n = 19) were also positive for other respiratory viruses. hMPV activity peaked in January and September in 2015; however, no seasonal pattern of hMPV infection was detected. Phylogenetic analyses of the N and F gene-fragments revealed that the hMPV strains circulating in Dhaka, Bangladesh, belonged to three genotypes: A2b, A2c, and B1. Genotype A (57%) was the predominant hMPV genotype circulating in Bangladesh during the study period. CONCLUSION: This study describes both the epidemiology of hMPV infection and its genotypic strain diversity in Dhaka, Bangladesh.
BACKGROUND: Acute respiratory infection (ARI) is a leading cause of morbidity and mortality in children in low and middle-income countries. Human metapneumovirus (hMPV) is one of the most common viral etiological agents for ARIs in children. OBJECTIVES: In this study, we explored the genotypic diversity and the epidemiology of hMPV among infants in Dhaka, Bangladesh. STUDY DESIGN: Between December 2014 and August 2016, a total of 3810 mid-turbinate nasal swab samples were collected from infants (0 to 6 months of age) who met clinical ARI criteria, as a part of a prospective ARI cohort study. hMPV was detected using polymerase chain reaction, and genotyped by sequencing and phylogenetic analysis. RESULTS:hMPV was identified in 206 (5.4%) nasal swab specimens. One-tenth of the hMPV-positive swabs (n = 19) were also positive for other respiratory viruses. hMPV activity peaked in January and September in 2015; however, no seasonal pattern of hMPVinfection was detected. Phylogenetic analyses of the N and F gene-fragments revealed that the hMPV strains circulating in Dhaka, Bangladesh, belonged to three genotypes: A2b, A2c, and B1. Genotype A (57%) was the predominant hMPV genotype circulating in Bangladesh during the study period. CONCLUSION: This study describes both the epidemiology of hMPVinfection and its genotypic strain diversity in Dhaka, Bangladesh.
Authors: Juan Carlos Muñoz-Escalante; Gabriel Mata-Moreno; Gerardo Rivera-Alfaro; Daniel E Noyola Journal: Viruses Date: 2022-05-16 Impact factor: 5.818