Literature DB >> 3036950

Rapid simultaneous assessment of neutrophil superoxide generation and lysosomal enzyme release.

B F Mandell, D Ohliger, J Rella.   

Abstract

A rapid method for the simultaneous measurement of neutrophil superoxide generation and beta-glucuronidase release is described. Assay of beta-glucuronidase using a fluorescent substrate is shown to be valid in the presence of reduced or unreduced ferricytochrome C, a prerequisite for the simultaneous assessment of this enzyme activity and O-2 generation.

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Year:  1987        PMID: 3036950     DOI: 10.1016/0022-1759(87)90191-8

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  2 in total

1.  Neutrophil actin dysfunction is a genetic disorder associated with partial impairment of neutrophil actin assembly in three family members.

Authors:  F S Southwick; G A Dabiri; T P Stossel
Journal:  J Clin Invest       Date:  1988-11       Impact factor: 14.808

2.  Natural inhibitor from Candida albicans blocks release of azurophil and specific granule contents by chemotactic peptide-stimulated human neutrophils.

Authors:  E H Smail; M P Kolotila; R Ruggeri; R D Diamond
Journal:  Infect Immun       Date:  1989-03       Impact factor: 3.441

  2 in total

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