Shanna Bastiaan-Net1, Marit Reitsma2,3, Jan H G Cordewener4, Johanna P M van der Valk5, Twan A H P America4, Anthony E J Dubois6, Roy Gerth van Wijk5, Huub F J Savelkoul7, Nicolette W de Jong5, Harry J Wichers2. 1. Wageningen Food and Biobased Research, Wageningen University and Research, Wageningen, The Netherlandsshanna.bastiaan@wur.nl. 2. Wageningen Food and Biobased Research, Wageningen University and Research, Wageningen, The Netherlands. 3. Food Chemistry, Wageningen University, Wageningen, The Netherlands. 4. Wageningen Plant Research, Wageningen University and Research, Wageningen, The Netherlands. 5. Section of Allergology, Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands. 6. GRIAC Research Institute, Department of Pediatric Pulmonology and Pediatric Allergology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. 7. Cell Biology and Immunology Group, Wageningen University, Wageningen, The Netherlands.
Abstract
BACKGROUND: Allergic sensitisation towards cashew nut often happens without a clear history of eating cashew nut. IgE cross-reactivity between cashew and pistachio nut is well described; however, the ability of cashew nut-specific IgE to cross-react to common tree nut species and other Anacardiaceae, like mango, pink peppercorn, or sumac is largely unknown. OBJECTIVES: Cashew nut allergic individuals may cross-react to foods that are phylogenetically related to cashew. We aimed to determine IgE cross-sensitisation and cross-reactivity profiles in cashew nut-sensitised subjects, towards botanically related proteins of other Anacardiaceae family members and related tree nut species. METHOD: Sera from children with a suspected cashew nut allergy (n = 56) were assessed for IgE sensitisation to common tree nuts, mango, pink peppercorn, and sumac using dot blot technique. Allergen cross-reactivity patterns between Anacardiaceae species were subsequently examined by SDS-PAGE and immunoblot inhibition, and IgE-reactive allergens were identified by LC-MS/MS. RESULTS: From the 56 subjects analysed, 36 were positive on dot blot for cashew nut (63%). Of these, 50% were mono-sensitised to cashew nuts, 19% were co-sensitised to Anacardiaceae species, and 31% were co-sensitised to tree nuts. Subjects co-sensitised to Anacardiaceae species displayed a different allergen recognition pattern than subjects sensitised to common tree nuts. In pink peppercorn, putative albumin- and legumin-type seed storage proteins were found to cross-react with serum of cashew nut-sensitised subjects in vitro. In addition, a putative luminal binding protein was identified, which, among others, may be involved in cross-reactivity between several Anacardiaceae species. CONCLUSIONS: Results demonstrate the in vitro presence of IgE cross-sensitisation in children towards multiple Anacardiaceae species. In this study, putative novel allergens were identified in cashew, pistachio, and pink peppercorn, which may pose factors that underlie the observed cross-sensitivity to these species. The clinical relevance of this widespread cross-sensitisation is unknown.
BACKGROUND:Allergic sensitisation towards cashew nut often happens without a clear history of eating cashew nut. IgE cross-reactivity between cashew and pistachio nut is well described; however, the ability of cashew nut-specific IgE to cross-react to common tree nut species and other Anacardiaceae, like mango, pink peppercorn, or sumac is largely unknown. OBJECTIVES:Cashew nut allergic individuals may cross-react to foods that are phylogenetically related to cashew. We aimed to determine IgE cross-sensitisation and cross-reactivity profiles in cashew nut-sensitised subjects, towards botanically related proteins of other Anacardiaceae family members and related tree nut species. METHOD: Sera from children with a suspected cashew nut allergy (n = 56) were assessed for IgE sensitisation to common tree nuts, mango, pink peppercorn, and sumac using dot blot technique. Allergen cross-reactivity patterns between Anacardiaceae species were subsequently examined by SDS-PAGE and immunoblot inhibition, and IgE-reactive allergens were identified by LC-MS/MS. RESULTS: From the 56 subjects analysed, 36 were positive on dot blot for cashew nut (63%). Of these, 50% were mono-sensitised to cashew nuts, 19% were co-sensitised to Anacardiaceae species, and 31% were co-sensitised to tree nuts. Subjects co-sensitised to Anacardiaceae species displayed a different allergen recognition pattern than subjects sensitised to common tree nuts. In pink peppercorn, putative albumin- and legumin-type seed storage proteins were found to cross-react with serum of cashew nut-sensitised subjects in vitro. In addition, a putative luminal binding protein was identified, which, among others, may be involved in cross-reactivity between several Anacardiaceae species. CONCLUSIONS: Results demonstrate the in vitro presence of IgE cross-sensitisation in children towards multiple Anacardiaceae species. In this study, putative novel allergens were identified in cashew, pistachio, and pink peppercorn, which may pose factors that underlie the observed cross-sensitivity to these species. The clinical relevance of this widespread cross-sensitisation is unknown.
Authors: A Diaz-Perales; C Collada; C Blanco; R Sanchez-Monge; T Carrillo; C Aragoncillo; G Salcedo Journal: J Allergy Clin Immunol Date: 1999-09 Impact factor: 10.793
Authors: Nicolette W de Jong; Severina Terlouw; Frank E van Boven; M S van Maaren; Marco W J Schreurs; Dianne B P M van den Berg-Somhorst; Diederik Esser; Shanna Bastiaan-Net Journal: Nutrients Date: 2021-04-18 Impact factor: 5.717
Authors: Christopher P Mattison; Barry Vant-Hull; Ana Cecilia Ribeiro de Castro; Heidi J Chial; Yvette Bren-Mattison; Peter J Bechtel; Edy Sousa de Brito Journal: Antibodies (Basel) Date: 2021-11-28
Authors: Shanna Bastiaan-Net; Maria C Pina-Pérez; Bas J W Dekkers; Adrie H Westphal; Antoine H P America; Renata M C Ariëns; Nicolette W de Jong; Harry J Wichers; Jurriaan J Mes Journal: Protein Sci Date: 2020-05-16 Impact factor: 6.725