Anastasia Kyselova1, Hanna Hinrichsmeyer2, Sven Zukunft1, Alexander W Mann3, Imke Dornauf3, Ingrid Fleming1, Voahanginirina Randriamboavonjy4. 1. Institute for Vascular Signalling, Centre of Molecular Medicine, Goethe University, Frankfurt am Main D-60596, Germany; German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany. 2. Institute for Vascular Signalling, Centre of Molecular Medicine, Goethe University, Frankfurt am Main D-60596, Germany. 3. Endokrinologikum Frankfurt, Frankfurt am Main D-60596, Germany. 4. Institute for Vascular Signalling, Centre of Molecular Medicine, Goethe University, Frankfurt am Main D-60596, Germany; German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany. Electronic address: voahangy@vrc.uni-frankfurt.de.
Abstract
BACKGROUND: Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disease associated with insulin resistance and increased risk of cardiovascular diseases. However, a biomarker for potential cardiovascular disease in PCOS patients is not available. MATERIALS AND METHODS: Twenty-two patients with PCOS and 22 healthy controls were included in the present study and amino acid profiling was performed on fasting plasma samples. Circulating microparticles were characterized by FACS analysis and complemented with enzyme activity assays. RESULTS: The ratio of ornithine to arginine was significantly increased in plasma form PCOS patients and was associated with a significant increase in plasma arginase levels and activity. Platelet-derived microparticles were identified to be the main sources of the increased plasma arginase activity. CONCLUSIONS: Increased levels of arginase-bearing platelet-derived microparticles contribute to the alteration of the arginine metabolism in patients with polycystic ovary syndrome. Moreover, ornithine and arginine levels represent early biomarkers of potential cardiovascular disease in PCOS patients.
BACKGROUND:Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disease associated with insulin resistance and increased risk of cardiovascular diseases. However, a biomarker for potential cardiovascular disease in PCOSpatients is not available. MATERIALS AND METHODS: Twenty-two patients with PCOS and 22 healthy controls were included in the present study and amino acid profiling was performed on fasting plasma samples. Circulating microparticles were characterized by FACS analysis and complemented with enzyme activity assays. RESULTS: The ratio of ornithine to arginine was significantly increased in plasma form PCOSpatients and was associated with a significant increase in plasma arginase levels and activity. Platelet-derived microparticles were identified to be the main sources of the increased plasma arginase activity. CONCLUSIONS: Increased levels of arginase-bearing platelet-derived microparticles contribute to the alteration of the arginine metabolism in patients with polycystic ovary syndrome. Moreover, ornithine and arginine levels represent early biomarkers of potential cardiovascular disease in PCOSpatients.
Authors: Ahmed Al-Qaissi; Saeed Alqarni; Zeeshan Javed; Stephen L Atkin; Thozhukat Sathyapalan; Rebecca V Vince; Leigh A Madden Journal: Endocrine Date: 2019-08-27 Impact factor: 3.633
Authors: ShanHu Gao; Fei Long; Zheng Jiang; Jun Shi; DongXue Ma; Yang Yang; Jin Bai; Ting-Li Han Journal: Front Physiol Date: 2022-09-20 Impact factor: 4.755