Arthur Cunha1, Paulo Nelson-Filho1, Guido Artemio Marañón-Vásquez1, Alice Gomes de Carvalho Ramos2, Beatriz Dantas2, Aline Monise Sebastiani3, Felipe Silvério3, Marjorie Ayumi Omori1, Amanda Silva Rodrigues4, Ellen Cardoso Teixeira5, Simone Carvalho Levy5, Marcelo Calvo de Araújo6, Mírian Aiko Nakane Matsumoto1, Fábio Lourenço Romano1, Lívia Azeredo A Antunes5, Delson João da Costa4, Rafaela Scariot7, Leonardo Santos Antunes5, Alexandre R Vieira8, Erika C Küchler9. 1. Department of Pediatric Dentistry, School of dentistry of Ribeirão Preto, University of São Paulo. Avenida do Café s/n - Campus da USP, Ribeirão Preto, SP, Brazil - CEP: 14040-904. 2. Department of Pediatric Dentistry, School of dentistry of Ribeirão Preto, University of São Paulo. Avenida do Café s/n - Campus da USP, Ribeirão Preto, SP, Brazil - CEP: 14040-904; Amazonian Education Institute. Rua Maceió 861, Adrianópolis, Manaus, AM, Brazil - CEP: 69057-010. 3. University. Rua Professor Pedro Viriato Parigot de Souza 5300 - Campo Comprido, Curitiba, PR, Brazil - CEP: 81200-452. 4. Professor, Department of Oral and Maxillofacial Surgery, Federal University of Paraná. Avenida Prefeito Lothário Meisser 632, Curitiba, PR, Brazil - CEP: 80210-170. 5. Program, School of Dentistry, Fluminense Federal University. Rua São Paulo 28, Campus do Valonguinho, Niterói, RJ, Brazil - CEP: 24020-150 and Rua Doutor Sílvio Henrique Braune 22, Nova Friburgo, RJ, Brazil - CEP: 28625-650. 6. Professor, Smile Graduate School and Clinic. Rua José Clemente 94, Centro, Niterói, RJ, Brazil. CEP: 24020-115. 7. Professor, Department of Oral and Maxillofacial Surgery, Federal University of Paraná. Avenida Prefeito Lothário Meisser 632, Curitiba, PR, Brazil - CEP: 80210-170; University. Rua Professor Pedro Viriato Parigot de Souza 5300 - Campo Comprido, Curitiba, PR, Brazil - CEP: 81200-452. 8. Department of Oral Biology, School of Dental Medicine, University of Pittsburgh. 412 Salk Pavilion, 335 Sutherland Street, Pittsburgh, PA, USA. 15261. 9. Department of Pediatric Dentistry, School of dentistry of Ribeirão Preto, University of São Paulo. Avenida do Café s/n - Campus da USP, Ribeirão Preto, SP, Brazil - CEP: 14040-904; University. Rua Professor Pedro Viriato Parigot de Souza 5300 - Campo Comprido, Curitiba, PR, Brazil - CEP: 81200-452. Electronic address: erika.kuchler@up.edu.br.
Abstract
OBJECTIVE: This study aimed to evaluate the association of genetic variants inACTN3 and MYO1H with craniofacial skeletal patterns in Brazilians. DESIGN: This cross-sectional study enrolled orthodontic and orthognathic patients selected from 4 regions of Brazil. Lateral cephalograms were used and digital cephalometric tracings and analyzes were performed for craniofacial phenotype determination. Participants were classified according to the skeletal malocclusion in Class I, II or III; and according to the facial type in Mesofacial, Dolichofacial or Brachyfacial. Genomic DNA was extracted from saliva samples containing exfoliated buccal epithelial cells and analyzed for genetic variants inACTN3 (rs678397 and rs1815739) and MYO1H (rs10850110) by real-time PCR. Chi-square or Fisher's exact tests were used for statistical analysis (α = 5%). RESULTS: A total of 646 patients were included in the present study. There was statistically significant association of the genotypes and/or alleles distributions with the skeletal malocclusion (sagittal skeletal pattern) and facial type (vertical pattern) for the variants assessed inACTN3 (P < 0.05). For the genetic variant evaluated in MYO1H, there was statistically significant difference between the genotypes frequencies for skeletal Class I and Class II (P < 0.05). The reported associations were different depending on the region evaluated. CONCLUSION: ACTN3 and MYO1H are associated with sagittal and vertical craniofacial skeletal patterns in Brazilian populations.
OBJECTIVE: This study aimed to evaluate the association of genetic variants inACTN3 and MYO1H with craniofacial skeletal patterns in Brazilians. DESIGN: This cross-sectional study enrolled orthodontic and orthognathic patients selected from 4 regions of Brazil. Lateral cephalograms were used and digital cephalometric tracings and analyzes were performed for craniofacial phenotype determination. Participants were classified according to the skeletal malocclusion in Class I, II or III; and according to the facial type in Mesofacial, Dolichofacial or Brachyfacial. Genomic DNA was extracted from saliva samples containing exfoliated buccal epithelial cells and analyzed for genetic variants inACTN3 (rs678397 and rs1815739) and MYO1H (rs10850110) by real-time PCR. Chi-square or Fisher's exact tests were used for statistical analysis (α = 5%). RESULTS: A total of 646 patients were included in the present study. There was statistically significant association of the genotypes and/or alleles distributions with the skeletal malocclusion (sagittal skeletal pattern) and facial type (vertical pattern) for the variants assessed inACTN3 (P < 0.05). For the genetic variant evaluated in MYO1H, there was statistically significant difference between the genotypes frequencies for skeletal Class I and Class II (P < 0.05). The reported associations were different depending on the region evaluated. CONCLUSION:ACTN3 and MYO1H are associated with sagittal and vertical craniofacial skeletal patterns in Brazilian populations.
Authors: Murilo Matias; Carlos Flores-Mir; Márcio Rodrigues de Almeida; Bruno da Silva Vieira; Karina Maria Salvatore de Freitas; Daniela Calabrese Nunes; Marcos Cezar Ferreira; Weber Ursi Journal: Korean J Orthod Date: 2021-11-25 Impact factor: 1.372
Authors: Bernardo Olsson; Mateus José da Silva; Camila Lago; Robson Diego Calixto; Lucas Alexandre Ramazzotto; Nelson Luis Barbosa Rebellato; Christian Kirschneck; Francisco Wanderley Garcia Paula-Silva; Erika Calvano Küchler; Rafaela Scariot Journal: Ann Maxillofac Surg Date: 2022-02-01
Authors: L de Frutos-Valle; C Martin; J A Alarcón; J C Palma-Fernández; R Ortega; A Iglesias-Linares Journal: Sci Rep Date: 2020-10-21 Impact factor: 4.379