Mohsen Moghoofei1, Shayan Mostafaei2,3, Abolfazl Nesaei4, Ashkan Etemadi5, Javid Sadri Nahand6, Hamed Mirzaei7, Bahman Rashidi8, Farhad Babaei1, Niloofar Khodabandehlou9. 1. Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran. 2. Department of Community Medicine, Faculty of Medicine, Alborz University of Medical Sciences, Alborz, Iran. 3. Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran. 4. Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran. 5. Department of Biology, Faculty of Science, Shahrekord University, Shahrekord, Iran. 6. Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. 7. Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashahd, Iran. 8. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 9. Department of Internal Medicine, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Thyroid cancer is a common endocrine malignancy whose incidence has increased in recent years. Several internal and external risk factors are involved in the development of this cancer, such as infectious agents. Evidence supporting the role of viral infection as an etiology for the invasiveness of thyroid cancer is increasing. The aim of this study was to determine the presence of the Epstein-Barr virus (EBV) and the association between viral gene products and thyroid tumor development. METHODS: Fifty-seven thyroid cancer specimens were collected from the same number of patients as well as 18 samples from healthy controls. The presence of the EBV genome and the genotyping was examined by polymerase chain reaction (PCR). Also, an enzyme-linked immunosorbent assay and real-time PCR were used to measure the expression levels of viral and cellular genes. RESULTS: The EBV DNA was detected in 71.9% of the samples, and it was also found that the presence of the EBV was associated with increasing development of thyroid tumor. CONCLUSION: Our results demonstrated that EBV infection may play a role in the development of thyroid tumor.
BACKGROUND:Thyroid cancer is a common endocrine malignancy whose incidence has increased in recent years. Several internal and external risk factors are involved in the development of this cancer, such as infectious agents. Evidence supporting the role of viral infection as an etiology for the invasiveness of thyroid cancer is increasing. The aim of this study was to determine the presence of the Epstein-Barr virus (EBV) and the association between viral gene products and thyroid tumor development. METHODS: Fifty-seven thyroid cancer specimens were collected from the same number of patients as well as 18 samples from healthy controls. The presence of the EBV genome and the genotyping was examined by polymerase chain reaction (PCR). Also, an enzyme-linked immunosorbent assay and real-time PCR were used to measure the expression levels of viral and cellular genes. RESULTS: The EBV DNA was detected in 71.9% of the samples, and it was also found that the presence of the EBV was associated with increasing development of thyroid tumor. CONCLUSION: Our results demonstrated that EBV infection may play a role in the development of thyroid tumor.
Authors: Janusz Klatka; Anna Hymos; Anna Szkatuła-Łupina; Ewelina Grywalska; Barbara Klatka; Michał Terpiłowski; Andrzej Stepulak Journal: In Vivo Date: 2019 Nov-Dec Impact factor: 2.155
Authors: Nabila El-Sheikh; Nahla O Mousa; Amany M Tawfeik; Alaa M Saleh; Iman Elshikh; Mohamed Deyab; Faten Ragheb; Manar M Moneer; Ahmed Kawashti; Ahmed Osman; Mohamed Elrefaei Journal: Breast Cancer (Auckl) Date: 2021-02-25