PURPOSE: A novel dissolution dynamic nuclear polarization (dDNP) polarizer platform is presented. The polarizer meets a number of key requirements for in vitro, preclinical, and clinical applications. METHOD: It uses no liquid cryogens, operates in continuous mode, accommodates a wide range of sample sizes up to and including those required for human studies, and is fully automated. RESULTS: It offers a wide operational window both in terms of magnetic field, up to 10.1 T, and temperature, from room temperature down to 1.3 K. The polarizer delivers a 13 C liquid state polarization for [1-13 C]pyruvate of 70%. The build-up time constant in the solid state is approximately 1200 s (20 minutes), allowing a sample throughput of at least one sample per hour including sample loading and dissolution. CONCLUSION: We confirm the previously reported strong field dependence in the range 3.35 to 6.7 T, but see no further increase in polarization when increasing the magnetic field strength to 10.1 T for [1-13 C]pyruvate and trityl. Using a custom dry magnet, cold head and recondensing, closed-cycle cooling system, combined with a modular DNP probe, and automation and fluid handling systems, we have designed a unique dDNP system with unrivalled flexibility and performance.
PURPOSE: A novel dissolution dynamic nuclear polarization (dDNP) polarizer platform is presented. The polarizer meets a number of key requirements for in vitro, preclinical, and clinical applications. METHOD: It uses no liquid cryogens, operates in continuous mode, accommodates a wide range of sample sizes up to and including those required for human studies, and is fully automated. RESULTS: It offers a wide operational window both in terms of magnetic field, up to 10.1 T, and temperature, from room temperature down to 1.3 K. The polarizer delivers a 13 C liquid state polarization for [1-13 C]pyruvate of 70%. The build-up time constant in the solid state is approximately 1200 s (20 minutes), allowing a sample throughput of at least one sample per hour including sample loading and dissolution. CONCLUSION: We confirm the previously reported strong field dependence in the range 3.35 to 6.7 T, but see no further increase in polarization when increasing the magnetic field strength to 10.1 T for [1-13 C]pyruvate and trityl. Using a custom dry magnet, cold head and recondensing, closed-cycle cooling system, combined with a modular DNP probe, and automation and fluid handling systems, we have designed a unique dDNP system with unrivalled flexibility and performance.
Authors: Tian Cheng; Adam P Gaunt; Irene Marco-Rius; Marcel Gehrung; Albert P Chen; Jacques J van der Klink; Arnaud Comment Journal: NMR Biomed Date: 2020-01-30 Impact factor: 4.044
Authors: Andreas B Schmidt; C Russell Bowers; Kai Buckenmaier; Eduard Y Chekmenev; Henri de Maissin; James Eills; Frowin Ellermann; Stefan Glöggler; Jeremy W Gordon; Stephan Knecht; Igor V Koptyug; Jule Kuhn; Andrey N Pravdivtsev; Francesca Reineri; Thomas Theis; Kolja Them; Jan-Bernd Hövener Journal: Anal Chem Date: 2022-01-01 Impact factor: 6.986
Authors: Stephan Berner; Andreas B Schmidt; Frowin Ellermann; Sergey Korchak; Eduard Y Chekmenev; Stefan Glöggler; Dominik von Elverfeldt; Jürgen Hennig; Jan-Bernd Hövener Journal: Phys Chem Chem Phys Date: 2021-01-28 Impact factor: 3.676
Authors: Ben J Tickner; Olga Semenova; Wissam Iali; Peter J Rayner; Adrian C Whitwood; Simon B Duckett Journal: Catal Sci Technol Date: 2020-01-28 Impact factor: 6.119