Gayan Wijesinghe1, Ayomi Dilhari1, Buddhika Gayani2,3, Nilwala Kottegoda2,4,3, Lakshman Samaranayake5, Manjula Weerasekera6. 1. Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka. 2. Department of Chemistry, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka. 3. Center for Advanced Materials Research, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka. 4. Institute of Nanotechnology, Center for Excellence in Nanotechnology, Nanoscience and Technology Park, Homagama, Sri Lanka. 5. Health Sciences Center, Kuwait University, Kuwait City, Kuwait. 6. Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka, mmweera@yahoo.com.
Abstract
OBJECTIVE: Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilm infections are notoriously difficult to manage. This study aimed at investigating the influence of four different culture media on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa and S. aureus. MATERIALS AND METHODS: We monitored four different culture media including Nutrient Broth, Brain Heart Infusion (BHI) broth, Luria-Bertani broth, and RPMI 1640 medium on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa (ATCC 27853) and S. aureus (ATCC 25923) using MTT assay and scanning electron microscopy (SEM). RESULTS: The most robust growth of the mono- and dual-species cultures was noted in BHI broth. On the contrary, RPMI 1640 medium promoted maximal initial adhesion of both the mono- and dual-species, but BHI broth fostered the maximal biofilm growth. SEM images showed profuse extracellular polysaccharide production in biofilms, particularly in coculture, in BHI medium. CONCLUSION: Our data demonstrate that BHI broth, relative to the other tested media, is the most conducive for in vitro evaluation of biofilm and planktonic growth kinetics of these two pathogens, both in mono- and coculture.
OBJECTIVE:Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilm infections are notoriously difficult to manage. This study aimed at investigating the influence of four different culture media on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa and S. aureus. MATERIALS AND METHODS: We monitored four different culture media including Nutrient Broth, Brain Heart Infusion (BHI) broth, Luria-Bertani broth, and RPMI 1640 medium on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa (ATCC 27853) and S. aureus (ATCC 25923) using MTT assay and scanning electron microscopy (SEM). RESULTS: The most robust growth of the mono- and dual-species cultures was noted in BHI broth. On the contrary, RPMI 1640 medium promoted maximal initial adhesion of both the mono- and dual-species, but BHI broth fostered the maximal biofilm growth. SEM images showed profuse extracellular polysaccharide production in biofilms, particularly in coculture, in BHI medium. CONCLUSION: Our data demonstrate that BHI broth, relative to the other tested media, is the most conducive for in vitro evaluation of biofilm and planktonic growth kinetics of these two pathogens, both in mono- and coculture.
Authors: S D Petinate; R M Martins; R R Coelho; M N Meirelles; M H Branquinha; A B Vermelho Journal: Mem Inst Oswaldo Cruz Date: 1999 Mar-Apr Impact factor: 2.743
Authors: Lisa Saiman; Bruce C Marshall; Nicole Mayer-Hamblett; Jane L Burns; Alexandra L Quittner; Debra A Cibene; Sarah Coquillette; Ann Yunker Fieberg; Frank J Accurso; Preston W Campbell Journal: JAMA Date: 2003-10-01 Impact factor: 56.272
Authors: Elena Y Trizna; Maria N Yarullina; Diana R Baidamshina; Anna V Mironova; Farida S Akhatova; Elvira V Rozhina; Rawil F Fakhrullin; Alsu M Khabibrakhmanova; Almira R Kurbangalieva; Mikhail I Bogachev; Airat R Kayumov Journal: Sci Rep Date: 2020-09-09 Impact factor: 4.379