Literature DB >> 3035196

Structure of the DNA gyrase-DNA complex as revealed by transient electric dichroism.

D C Rau, M Gellert, F Thoma, A Maxwell.   

Abstract

We have analyzed the structure of complexes between DNA gyrase and four defined DNA fragments by electric dichroism. Both the extrapolated dichroism and relaxation time of these complexes suggest that a single turn of DNA is wrapped around the enzyme with the entry and exit points located close together. The average angle between the DNA tails emerging from the particle is about 120 degrees. This structure is consistent with that seen by electron microscopy. Addition of ATP or the non-hydrolyzable ATP analog 5'-adenylyl-beta, gamma-imidodiphosphate results in a structural change of the complex, consistent with the DNA tails now being wrapped around the protein. The significance of these observations with respect to the mechanism of DNA supercoiling by DNA gyrase is discussed.

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Year:  1987        PMID： 3035196     DOI： 10.1016/0022-2836(87)90266-x

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  28 in total

1.  A model for the mechanism of strand passage by DNA gyrase.

Authors:  S C Kampranis; A D Bates; A Maxwell
Journal:  Proc Natl Acad Sci U S A       Date:  1999-07-20       Impact factor: 11.205

2.  The cleavage of DNA at phosphorothioate internucleotidic linkages by DNA gyrase.

Authors:  S T Dobbs; P M Cullis; A Maxwell
Journal:  Nucleic Acids Res       Date:  1992-07-25       Impact factor: 16.971

3.  The C-terminal domain of DNA gyrase A adopts a DNA-bending beta-pinwheel fold.

Authors:  Kevin D Corbett; Ryan K Shultzaberger; James M Berger
Journal:  Proc Natl Acad Sci U S A       Date:  2004-05-03       Impact factor: 11.205

4.  Proteolysis patterns of epitopically labeled yeast DNA topoisomerase II suggest an allosteric transition in the enzyme induced by ATP binding.

Authors:  J E Lindsley; J C Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-01       Impact factor: 11.205

5.  DNA gyrase can cleave short DNA fragments in the presence of quinolone drugs.

Authors:  M E Cove; A P Tingey; A Maxwell
Journal:  Nucleic Acids Res       Date:  1997-07-15       Impact factor: 16.971

6.  The C-terminal domain of the Escherichia coli DNA gyrase A subunit is a DNA-binding protein.

Authors:  R J Reece; A Maxwell
Journal:  Nucleic Acids Res       Date:  1991-04-11       Impact factor: 16.971

7.  In the presence of subunit A inhibitors DNA gyrase cleaves DNA fragments as short as 20 bp at specific sites.

Authors:  H Gmünder; K Kuratli; W Keck
Journal:  Nucleic Acids Res       Date:  1997-02-01       Impact factor: 16.971

Review 8.  DNA gyrase, topoisomerase IV, and the 4-quinolones.

Authors:  K Drlica; X Zhao
Journal:  Microbiol Mol Biol Rev       Date:  1997-09       Impact factor: 11.056

Review 9.  Biochemical characteristics and physiological significance of major DNA topoisomerases.

Authors:  J A Sutcliffe; T D Gootz; J F Barrett
Journal:  Antimicrob Agents Chemother       Date:  1989-12       Impact factor: 5.191

10.  DNA gyrase binds to the family of prokaryotic repetitive extragenic palindromic sequences.

Authors:  Y Yang; G F Ames
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205
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