| Literature DB >> 3034861 |
Y C Sung, D Parsell, P M Anderson, J A Fuchs.
Abstract
The gene in Escherichia coli for cyanase, designated cynS, was localized to a BglII restriction site approximately 1.7 kilobases from the lacA end of the lac operon. The gene was cloned into the pUC13 vector. Maxicell analysis of plasmid-encoded proteins confirmed that the BglII site is in the region encoding the structural gene for cyanase. Cyanase-deficient strains had increased sensitivity to cyanate and were not able to use cyanate as a nitrogen source.Entities:
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Year: 1987 PMID: 3034861 PMCID: PMC212143 DOI: 10.1128/jb.169.6.2639-2642.1987
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490