Literature DB >> 3034856

Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions.

C W Ronson, B T Nixon, L M Albright, F M Ausubel.   

Abstract

We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.

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Year:  1987        PMID: 3034856      PMCID: PMC212082          DOI: 10.1128/jb.169.6.2424-2431.1987

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  39 in total

1.  Studies on the regulation and function of the Klebsiella pneumoniae ntrA gene.

Authors:  M J Merrick; W D Stewart
Journal:  Gene       Date:  1985       Impact factor: 3.688

2.  Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.

Authors:  R Staden
Journal:  Nucleic Acids Res       Date:  1982-08-11       Impact factor: 16.971

3.  Two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and ntrC.

Authors:  B T Nixon; C W Ronson; F M Ausubel
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

4.  Nucleotide sequence surrounding transcription initiation site of xylABC operon on TOL plasmid of Pseudomonas putida.

Authors:  S Inouye; Y Ebina; A Nakazawa; T Nakazawa
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

5.  Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants.

Authors:  A M Friedman; S R Long; S E Brown; W J Buikema; F M Ausubel
Journal:  Gene       Date:  1982-06       Impact factor: 3.688

6.  glnF-lacZ fusions in Escherichia coli: studies on glnF expression and its chromosomal orientation.

Authors:  I Castaño; F Bastarrachea
Journal:  Mol Gen Genet       Date:  1984

7.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

Authors:  G Ditta; S Stanfield; D Corbin; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

8.  Structural relationships among Rhizobium meliloti symbiotic promoters.

Authors:  M Better; B Lewis; D Corbin; G Ditta; D R Helinski
Journal:  Cell       Date:  1983-12       Impact factor: 41.582

9.  Nucleotide sequence and transcriptional initiation site of two Pseudomonas aeruginosa pilin genes.

Authors:  K Johnson; M L Parker; S Lory
Journal:  J Biol Chem       Date:  1986-11-25       Impact factor: 5.157

10.  Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.

Authors:  M Drummond; P Whitty; J Wootton
Journal:  EMBO J       Date:  1986-02       Impact factor: 11.598

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  114 in total

1.  Isolation and characterization of the nifUSVW-rpoN gene cluster from Rhodobacter sphaeroides.

Authors:  W G Meijer; F R Tabita
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

2.  Purification and characterization of the AAA+ domain of Sinorhizobium meliloti DctD, a sigma54-dependent transcriptional activator.

Authors:  Hao Xu; Baohua Gu; B Tracy Nixon; Timothy R Hoover
Journal:  J Bacteriol       Date:  2004-06       Impact factor: 3.490

3.  Mutations in the two flagellin genes of Rhizobium meliloti.

Authors:  K Bergman; E Nulty; L H Su
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

4.  Novel transcriptional control of the pyruvate formate-lyase gene: upstream regulatory sequences and multiple promoters regulate anaerobic expression.

Authors:  G Sawers; A Böck
Journal:  J Bacteriol       Date:  1989-05       Impact factor: 3.490

5.  Characterization of a novel Azorhizobium caulinodans ORS571 two-component regulatory system, NtrY/NtrX, involved in nitrogen fixation and metabolism.

Authors:  K Pawlowski; U Klosse; F J de Bruijn
Journal:  Mol Gen Genet       Date:  1991-12

6.  Identification and sequence analysis of the Rhizobium meliloti dctA gene encoding the C4-dicarboxylate carrier.

Authors:  T Engelke; D Jording; D Kapp; A Pühler
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

7.  Identification of a gene linked to Rhizobium meliloti ntrA whose product is homologous to a family to ATP-binding proteins.

Authors:  L M Albright; C W Ronson; B T Nixon; F M Ausubel
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490

Review 8.  Genetic regulation of nitrogen fixation in rhizobia.

Authors:  H M Fischer
Journal:  Microbiol Rev       Date:  1994-09

9.  Cloning and characterization of the sigA gene encoding the major sigma subunit of Rhizobium meliloti.

Authors:  B G Rushing; S R Long
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490

10.  Aerobic inactivation of Rhizobium meliloti NifA in Escherichia coli is mediated by lon and two newly identified genes, snoB and snoC.

Authors:  E Huala; A L Moon; F M Ausubel
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

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