Association Between Anxiety and Depression and Gastroesophageal Reflux Disease: Results From a Large Cross-sectional Study.

BACKGROUND/AIMS: The different clinical manifestations of gastroesophageal reflux disease (GERD) may be influenced by associated psychological factors. We evaluated the psychological status (anxiety and depression) according to each subtype of GERD.
METHODS: Subjects who underwent esophagogastroduodenoscopy and completed a symptom questionnaire between January 2008 and December 2011 were analyzed. The subjects were classified into the following groups: erosive reflux disease (ERD), non-erosive reflux disease (NERD), asymptomatic erosive esophagitis (AEE), and controls. Anxiety and depression were assessed using the State-Trait Anxiety Inventory and Beck Depression Inventory, respectively.
RESULTS: We analyzed 19 099 subjects: 16 157 (84.6%), 176 (0.9%), 1398 (7.3%), and 1368 (7.2%) in the control, ERD, NERD, and AEE groups, respectively. Multiple multinomial logistic regression revealed a significant association of increased state (adjusted OR, 1.89; 95% CI, 1.53-2.33) and trait anxiety (adjusted OR, 1.78; 95% CI, 1.34-2.35) and depression (adjusted OR, 2.21; 95% CI, 1.75-2.80) with NERD. ERD group showed a significant association only with state anxiety (adjusted OR, 2.20; 95% CI, 1.27-3.81) and depression (adjusted OR, 2.23; 95% CI, 1.18-4.22). The AEE group, however, did not show any significant association with psychological factors.
CONCLUSION: This cross-sectional study revealed that anxiety and depression levels were significantly higher in subjects with GERD (notably in the NERD) than in controls.

Introduction

Gastroesophageal reflux disease (GERD) is defined as a condition in which gastric contents reflux to the esophagus, causing troublesome symptoms such as heartburn and acid regurgitation.1 GERD is one of the most prevalent gastrointestinal disorders globally, showing an increasing prevalence in several developing countries.2,3 This disorder runs a chronic course, leads to a considerable deterioration in the quality of life of patients, and is associated with a high economic burden worldwide.4–6 Furthermore, in a few patients, GERD may progress and cause severe complications such as strictures, Barrett’s esophagus, and esophageal adenocarcinoma.7 Thus, GERD is considered a major health concern in most countries.

In approximately 33–50% of patients with typical GERD symptoms, endoscopic examination reveals distal esophageal abnormalities such as erosions or ulcers, whereas the remaining patients do not show any endoscopic abnormalities.8,9 Thus, GERD is categorized into 2 subtypes based on endoscopic findings: erosive reflux disease (ERD) and non-erosive reflux disease (NERD). However, a few patients show endoscopic evidence of esophageal mucosal erosions/ulcers typically indicating erosive esophagitis (EE), although they remain asymptomatic and are therefore categorized into an asymptomatic EE (AEE) group. To date, it is unclear why a few patients with EE present with symptoms and others do not.

Many studies have investigated the relationship between functional gastrointestinal disorder and psychological factors.10,11 A close relationship has been established between the brain and the gastrointestinal tract. For example, stress and emotions can affect gastrointestinal function, as well as the occurrence of gastrointestinal symptoms and disease. Likewise, the state of the gastrointestinal organs may affect a person’s emotional status. Psychological factors may influence the severity of a functional gastrointestinal disorder by affecting the perception of pain through an action on the gut-brain axis—a concept that is also applicable to patients with GERD. Additionally, when psychological factors accompany this condition, the treatment of the functional gastrointestinal disorder becomes difficult, which contributes to poor outcomes.10 To date, a few studies describing GERD have shown that psychological factors, particularly anxiety and depression, play an important role in patients with GERD; however, the results of those studies have been inconsistent.12–17 Many studies have reported a significant association of anxiety with GERD; however anxiety was not associated with the risk of GERD in a 10-year follow-up study in Sweden.15 Furthermore, in 1 prospective observational cohort study, high anxiety levels were not associated with the number of reflux symptoms, but only showed a relation to the severity of some reflux symptoms such as retrosternal pain or burning.14 In addition, some studies failed to demonstrate a significant association between depression and GERD.12,14–16 Moreover, only a few studies have analyzed the differences in the effects of psychological factors among the subtypes of GERD.12,17

Given this background, we examined the risk factors, particularly the psychological factors, associated with the subtypes of GERD in Koreans who underwent a health check-up. A clear understanding of the association between GERD and psychological factors is useful for administering optimal treatment in subjects with GERD because psychological factors can exacerbate GERD symptoms, worsen treatment outcomes, and interfere with the quality of life.

Materials and Methods

Subjects and Study Design

We performed a retrospective cross-sectional study that included 27 906 subjects who underwent upper gastrointestinal endoscopy and completed a symptom questionnaire on the same day for routine health checkup at the Seoul National University Hospital Healthcare System Gangnam Center between January 2008 and December 2011. A schematic protocol of the study design is illustrated in Figure 1. We excluded 932 subjects from the analysis based on the following criteria: a diagnosis of gastroesophageal carcinoma or active peptic ulcer based on upper gastrointestinal endoscopy findings, and a history of gastrectomy. In addition, we excluded the subjects with symptoms of functional gastrointestinal disorder other than reflux from the control group, which could lead to bias in the results of the study. Eventually, 19 099 subjects were included in the study, and their clinical and endoscopic records were reviewed. The study protocol was approved by the ethics committee of Seoul National University Hospital (institutional review board No. H-1510-046-710). Written informed consent was obtained from all subjects before every procedure.

Figure 1

Study population. EGD, esophagogastroduodenoscopy; FGID, functional gastrointestinal disorder; AEE, asymptomatic erosive esophagitis; NERD, non-erosive reflux disease; ERD, erosive reflux disease.

Clinical and Laboratory Evaluations

All subjects completed a structured, self-administered questionnaire about basic demographic characteristics, general health and lifestyle, medical history, and gastrointestinal symptoms. Height and body weight were measured using a digital scale. The body mass index (BMI) was calculated as weight (kg)/height2 (m2). Based on smoking status, subjects were categorized as current smoker or non-current smoker (former or never smoker). Based on alcohol consumption, subjects were categorized as alcohol user or non-user (does not drink any alcohol). Laboratory evaluation was performed on the same day, including serum Helicobacter pylori IgG antibody test (H. pylori-EIA-Well kit; Radim, Rome, Italy), for determining the presence of H. pylori infection.

Endoscopic Examinations

All endoscopic examinations were performed by 17 board-certified gastroenterologists. Conventional white light endoscopes (Olympus GIF-H260 and GIF-H290 series; Olympus Optical Co, Tokyo, Japan) were used for all procedures. In subjects who showed macroscopic findings of EE, atrophic gastritis, and intestinal metaplasia, the severity of the lesions was assessed and graded at each endoscopic examination. Atrophic gastritis was defined as endoscopically proven thinning of the gastric mucosa in the antrum or the body, with transparent submucosal blood vessels. Intestinal metaplasia was defined as ash-colored nodular change observed endoscopically.

Definition of Gastroesophageal Reflux Disease and Its Subtypes

Before undergoing the endoscopic examination, all subjects submitted themselves to a medical interview by physicians for the evaluation of gastrointestinal symptoms. The findings were recorded in a symptoms checklist. In the present study, based on the Montreal definition, we defined GERD as the presence of heartburn (burning sensation in the retrosternal area) and/or acid regurgitation (perception of reflux of stomach contents into the mouth or the hypopharynx) occurring at least once a week.1 Among subjects with GERD, based on the Los Angeles classification of esophagitis, those with endoscopically proven esophageal mucosal erosions/ulcers were classified as the ERD group, and the remaining subjects were classified as the NERD group. Those with esophageal mucosal erosions/ulcers without reflux symptoms were categorized as the AEE group. Those without reflux symptoms and with normal endoscopic findings were categorized as the control group.

The study did not include the results of esophageal impedance-pH monitoring tests or the response to proton pump inhibitors (PPIs). Therefore, subjects included in the NERD group may have been markedly heterogeneous, such as functional heartburn, acid hypersensitive esophagus, and non-acid hypersensitive esophagus.18,19 Patients with functional heartburn, in particular, were reported to have higher levels of anxiety than patients with GERD, and the inclusion of functional heartburn patients in the NERD group may exaggerate the potential association between NERD and psychological factors.14 Careful interpretation of the results is warranted.

Evaluation of the State of Anxiety and Depression

The State-Trait Anxiety Inventory (STAI) scale was used to assess the level of anxiety in all subjects.20 The STAI is a well-known psychological instrument comprising 2 self-report rating scales with 20 items each, for the measurement of 2 types of anxiety: state anxiety (how one feels at the moment, STAI-X1) and trait anxiety (how one generally feels, STAI-X2). Each item is rated between 1 and 4 depending on the frequency of target complaints (never, sometimes, often, and always), and overall scores are obtained by summing the ratings for the items (range, 20–80). We considered subjects showing moderate-to-severe state or trait anxiety by using a cut-off value of STAI-X1 ≥57 or STAI-X2 ≥59, respectively.

The depression status of subjects was evaluated using the Beck Depression Inventory (BDI) scale, one of the most commonly used self-report instruments designed to detect and measure the severity of depression in the general population.21 The BDI comprises of 21 items describing symptoms and attitudes to depression, and each item is rated between 0 and 3 in terms of intensity. The total score ranges between 0 and 63—the higher the score, the greater the degree of depression. By using the cutoff value of 15, subjects were classified as having no-to-mild depression (BDI < 15) or moderate-to-severe depression (BDI ≥ 15).22

Statistical Methods

All data were expressed as means ± SD or as numbers (percentages). The means of continuous variables were compared using one-way ANOVA, and categorical variables were compared using the chi-square test. Differences in anxiety or depression levels among GERD subtypes were analyzed using one-way ANOVA with Tukey’s honestly significant difference post hoc analysis. Multinomial logistic regression analysis was used to evaluate the clinical characteristics of the subjects in the 4 groups. Univariate multinomial logistic regression was used to screen for important clinical parameters. Variables showing a P-value of < 0.05 from univariate multinomial logistic regression were included in multiple multinomial logistic regression. ORs and 95% CIs were used to estimate the association between various clinical and psychological characteristics and the GERD subtypes. A P-value of < 0.05 was considered statistically significant. Statistical analyses were performed using R software, version 3.4.3 (R Foundation for Statistical Computing, Vienna, Austria). For multinomial logistic regression analysis, we used the Vector Generalized Additive Models package for R.

Results

Characteristics of the Study Subjects

This study included 19 099 subjects, among whom 1574 (8.2%) had reflux symptoms. Subjects with reflux symptoms were classified into the ERD (176, 0.9%) and NERD (1398, 7.3%) groups according to the endoscopic findings of the gastroesophageal junction. Among 19 099 subjects, 1368 (7.2%) had endoscopically proven esophageal mucosal erosions/ulcers but did not have any reflux symptoms, and they were classified as the AEE group. The remaining subjects (16 157, 84.6%) were classified as the control group. The baseline demographic and clinical characteristics of the study subjects are shown in Table 1. Subjects in the ERD and NERD groups were significantly younger than those in the control group (45.8 ± 10.9 years and 47.0 ± 11.5 years vs 48.9 ± 11.1 years, P < 0.001, respectively). The percentage of women was lower in the ERD and AEE groups than in the control group (14.2% and 11.5% vs 37.3%, P < 0.001, respectively), but higher in the NERD group than in the control group (42.4% vs 37.3%, P < 0.001). BMI was significantly higher in the ERD and AEE groups than in the control group (25.5 ± 3.2 and 25.1 ± 3.0 vs 23.6 ± 3.0, P < 0.001, respectively). When compared to the control group, the percentage of subjects with diabetes was significantly higher in the AEE group and significantly lower in the NERD group (P < 0.001, all). The percentage of current smokers and alcohol user was significantly higher in the ERD group and the NERD group than in the control group (P < 0.001, all). The percentage of married subjects was significantly lower in the 2 subgroups showing reflux symptoms (ERD and NERD groups) than in the control group (P < 0.001). Compared with the control group, the percentage of those who used aspirin showed a statistically significant difference only in the AEE group (P < 0.001). The percentage of NSAIDs users was not significantly different from the control group in all 3 subtypes. The H. pylori seropositivity rates and incidence of endoscopic atrophic gastritis were significantly lower in the 3 subtypes than in the control group, whereas the incidence of intestinal metaplasia was significantly lower in the NERD group and AEE group than in the control group.

Table 1

Baseline Characteristics of Different Subgroups of Gastroesophageal Reflux Disease

	Control (n = 16 157)	ERD (n = 176)			NERD (n = 1398)			AEE (n = 1368)
			OR (95% CI)	P-value		OR (95% CI)	P-value		OR (95% CI)	P-value
Age (yr)	48.9 ± 11.1	45.8 ± 10.9	0.97 (0.96–0.99)	< 0.001	47.0 ± 11.5	0.98 (0.98–0.99)	< 0.001	48.5 ± 11.1	1.00 (0.99–1.00)	0.224
Sex (female)	6029 (37.3)	25 (14.2)	0.28 (0.18–0.43)	< 0.001	593 (42.4)	1.24 (1.11–1.38)	< 0.001	158 (11.5)	0.22 (0.18–0.26)	< 0.001
BMI (kg/m2)	23.6 ± 3.0	25.5 ± 3.2	1.21 (1.16–1.27)	< 0.001	23.7 ± 3.2	1.01 (0.99–1.03)	0.290	25.1 ± 3.0	1.17 (1.15–1.19)	< 0.001
Questionnaire
Diabetes history	911 (5.6)	6 (3.4)	0.59 (0.26–1.34)	0.207	49 (3.5)	0.61 (0.45–0.81)	< 0.001	107 (7.8)	1.42 (1.15–1.75)	< 0.001
Current smoking	8179 (50.6)	138 (78.4)	3.54 (2.47–5.08)	< 0.001	730 (52.2)	1.07 (0.96–1.19)	0.252	1021 (74.6)	2.87 (2.53–3.25)	< 0.001
Alcohol use	11 272 (69.8)	153 (86.9)	2.88 (1.86–4.47)	< 0.001	961 (68.7)	0.95 (0.85–1.07)	0.424	1170 (85.5)	2.56 (2.20–2.99)	< 0.001
Marriage status	14 882 (92.1)	144 (81.8)	0.39 (0.26–0.57)	< 0.001	1215 (86.9)	0.57 (0.48–0.67)	< 0.001	1253 (91.6)	0.93 (0.77–1.14)	0.498
Aspirin use	1908 (11.8)	15 (8.5)	0.70 (0.41–1.18)	0.181	156 (11.2)	0.94 (0.79–1.12)	0.469	206 (15.1)	1.32 (1.13–1.55)	< 0.001
NSAID use	1190 (7.4)	7 (4.0)	0.52 (0.24–1.11)	0.092	108 (7.7)	1.05 (0.86–1.29)	0.622	116 (8.5)	1.17 (0.96–1.42)	0.132
Helicobacter pylori
Seropositive	8593 (53.2)	44 (25.0)	0.29 (0.21–0.41)	< 0.001	692 (49.5)	0.86 (0.77–0.96)	0.008	393 (28.7)	0.35 (0.31–0.40)	< 0.001
Endoscopic findings
Atrophic gastritis	5288 (32.7)	30 (17.0)	0.42 (0.29–0.63)	< 0.001	363 (26.0)	0.72 (0.64–0.82)	< 0.001	345 (25.2)	0.69 (0.61–0.79)	< 0.001
Intestinal metaplasia	2484 (10.3)	12 (6.8)	0.60 (0.33–1.07)	0.085	105 (7.5)	0.66 (0.54–0.81)	< 0.001	101 (7.4)	0.65 (0.53–0.80)	< 0.001

ERD, erosive reflux disease; NERD, non-erosive reflux disease; AEE, asymptomatic erosive esophagitis; BMI, body mass index.

Values are presented as mean ± SD or n (%).

Relationship Between Psychological Status and Gastroesophageal Reflux Disease

The contribution of psychological factors associated with each subtype of GERD is shown in Figure 2. The level of state anxiety was significantly higher in the NERD group (P < 0.001) and the ERD group (P = 0.002) than in the control group, whereas the AEE group showed no significant difference from the control group. The level of trait anxiety was significantly different only in the NERD group among the subtypes of GERD (P < 0.001). Concerning depression level, there was a significant difference between the ERD and control (P < 0.001), NERD and control (P < 0.001), and the AEE and control groups (P = 0.001). In addition, significant differences were also observed among the 3 subtypes (NERD > ERD > AEE > control). The percentage of subjects who exceeded the cutoff value for each psychological score (ie, those presenting with moderate-to-severe psychological factors) showed the same pattern as the aforementioned comparison using the mean value (data not shown).

Figure 2

Psychological scores of different subtypes of gastroesophageal reflux disease (GERD). (A) Scores of state anxiety (State-Trait Anxiety Inventory [STAI]-X1) (B) Scores of trait anxiety (STAI-X2) (C) Scores of depression (Beck Depression Inventory [BDI]). ERD, erosive reflux disease; NERD, non-erosive reflux disease; AEE, asymptomatic erosive esophagitis.

Factors Associated With Each Subtype of Gastroesophageal Reflux Disease

The results of the multinomial logistic regression analysis are presented in Table 2. Female sex was observed to be an independent associated factor for NERD, and male sex for AEE. High BMI and current smoking were independently associated with all 3 subtypes of GERD. Alcohol use was associated only with the AEE group among the 3 subtypes of GERD. Marital status showed a significant association with ERD and NERD. The presence of diabetes mellitus showed a significant inverse correlation with NERD. An H. pylori-seropositive status and endoscopically proven atrophic gastritis also showed significant inverse correlations with the ERD and AEE subtypes, whereas the presence of intestinal metaplasia showed a significant inverse correlation with the NERD subtype.

Table 2

Multinomial Logistic Regression Analysis of Variables Associated With Subtypes of Gastroesophageal Reflux Disease

	Control	ERD		NERD		AEE
		OR (95% CI)	P-value	OR (95% CI)	P-value	OR (95% CI)	P-value
Age	1.00	1.01 (0.99–1.02)	0.495	1.00 (0.99–1.00)	0.157	1.00 (1.00–1.01)	0.524
Sex (female)	1.00	0.68 (0.39–1.18)	0.168	1.42 (1.20–1.69)	< 0.001	0.38 (0.31–0.48)	< 0.001
BMI	1.00	1.18 (1.13–1.24)	< 0.001	1.04 (1.02–1.06)	< 0.001	1.12 (1.09–1.14)	< 0.001
Current smoking	1.00	2.28 (1.47–3.55)	< 0.001	1.37 (1.18–1.59)	< 0.001	1.46 (1.26–1.70)	< 0.001
Alcohol use	1.00	1.44 (0.88–2.34)	0.144	0.96 (0.83–1.10)	0.531	1.25 (1.05–1.49)	0.012
Marriage status	1.00	0.45 (0.27–0.74)	0.002	0.71 (0.58–0.87)	< 0.001	0.96 (0.76–1.22)	0.728
Diabetes mellitus	1.00	0.51 (0.22–1.18)	0.116	0.65 (0.48–0.87)	0.004	1.13 (0.91–1.41)	0.269
Aspirin use	1.00	0.80 (0.43–1.49)	0.480	1.00 (0.80–1.25)	0.993	1.11 (0.91–1.36)	0.310
NSAID use	1.00	0.59 (0.25–1.40)	0.230	1.19 (0.92–1.53)	0.180	0.93 (0.73–1.19)	0.567
Helicobacter pylori seropositivity	1.00	0.32 (0.23–0.46)	< 0.001	0.96 (0.86–1.08)	0.540	0.36 (0.31–0.40)	< 0.001
Atrophic gastritis	1.00	0.55 (0.35–0.87)	0.010	0.88 (0.76–1.02)	0.084	0.80 (0.69–0.93)	0.003
Intestinal metaplasia	1.00	1.10 (0.58–2.11)	0.763	0.79 (0.64–0.99)	0.038	0.84 (0.67–1.06)	0.147
State anxiety	1.00	2.20 (1.27–3.81)	0.005	1.89 (1.53–2.33)	< 0.001	1.25 (0.96–1.65)	0.103
Trait anxiety	1.00	1.07 (0.44–2.59)	0.882	1.78 (1.34–2.35)	< 0.001	1.27 (0.83–1.96)	0.270
Depression	1.00	2.23 (1.18–4.22)	0.013	2.21 (1.75–2.80)	< 0.001	1.29 (0.93–1.80)	0.132

ERD, erosive reflux disease; NERD, non-erosive reflux disease; AEE, asymptomatic erosive esophagitis; BMI, body mass index.

Among the 3 GERD subtypes, NERD showed a significant association with all 3 psychological statuses examined in this study. NERD had a significant association with state anxiety (adjusted OR, 1.89; 95% CI, 1.53–2.33) and trait anxiety (adjusted OR, 1.78; 95% CI, 1.34–2.35). The NERD group also had a significant association with depression (adjusted OR, 221; 95% CI, 1.75–2.80). However, only state anxiety and depression showed a significant correlation with ERD, and psychological status did not show any correlation with AEE.

Discussion

In this study, we investigated the association between psychological status, such as anxiety and depression, and the clinical manifestations of GERD in the Korean population. After adjusting for various clinical factors, subjects in the ERD group showed higher state anxiety and depression levels than those in the control group. Subjects in the NERD group showed higher levels of all 3 psychological conditions (ie, state anxiety, trait anxiety, and depression) than those in the control group. Psychological factors were observed to be not associated with AEE. Thus, anxiety and depression levels were significantly higher in subjects with GERD, most notably in the NERD group.

Concerning the baseline characteristics, male sex was associated with the AEE group and the female sex was associated with the NERD group, which was in agreement with the results of previous studies.23–28 A high BMI was identified as a common associated factor among all 3 subgroups of GERD in this study. Prior studies have reported a positive correlation between BMI and disease prevalence in ERD and AEE.23,24,28 Obesity causes an increase in intragastric pressure, and a consequently elevated gastroesophageal pressure gradient leads to the development of hiatal hernia, which is known to be significantly associated with esophagitis.29 However, although several studies have been performed, the association between NERD and BMI has not yet been conclusively established.23,24,30 In this study, a H. pylori-seropositive status was inversely correlated with both EE with and without reflux symptoms, but not with NERD—findings that are in agreement with previous studies.23,24,30,31

Approximately 50% of persons with typical reflux symptoms present with an associated endoscopically proven abnormality, and approximately 50% of those with typical reflux-related esophagitis are asymptomatic.25,31 This discrepancy between the patients’ symptom perception and the degree of endoscopically identified mucosal injury suggests that other factors in addition to the pathogenesis of GERD may influence its clinical manifestations, and the psychological aspect is considered one such determinant.

In the current study, state and trait anxiety and depression levels were compared between controls and each subgroup of GERD. The results of this study demonstrated that the levels of both anxiety and depression were higher in subjects with GERD than in controls. A detailed analysis of the subtypes of GERD showed a significant association of state and trait anxiety and depression with NERD. Only state anxiety and depression showed a significant association with ERD. However, no significant difference was observed in the level of anxiety and depression in the AEE compared with the control group.

Previous studies that examined the association between GERD and psychological factors reported that anxiety was significantly associated with GERD, and the results of this current study were in agreement with those findings.12–14,17,32 The association between the clinical manifestations of GERD and psychological factors such as anxiety and depression can be explained by the following mechanism. Psychological factors precede the manifestations of GERD. Certain psychological conditions such as anxiety may directly promote acid reflux by lowering the pressure of the lower esophageal sphincter, changing esophageal motility, or increasing gastric acid secretion.33,34 The mechanism by which psychological factors influence reflux symptoms has also been demonstrated in animal studies. Rats subjected to psychological stress showed disruption of the tight junctions of the esophageal epithelium with a consequent weakening/lowering of the barrier function of the esophageal mucosa, thereby increasing its vulnerability to reflux.35 Moreover, anxiety and depression can lead to hypochondriasis, which indirectly lowers the threshold of reflux perception and exaggerates the sensation of reflux symptoms compared to that observed in controls.36,37 In a previous study, anxiety and depression levels did not significantly affect the acid exposure time and the number of reflux episodes, although the severity of reflux symptoms showed a significant association with anxiety levels in patients with GERD.14 Conversely, reflux symptoms may cause anxiety and depression. Persistent reflux symptoms lead to distress and may precipitate anxiety and depression. Previous studies have shown that patients, particularly those with a poor response to PPIs, were more likely to show higher levels of anxiety and depression, and these partial PPI responders were most common in the NERD group among the subtypes of GERD.38 Therefore, the relationship between anxiety, depression, and GERD involves a complex interplay of various mechanisms, and a multidisciplinary approach is needed to understand this relationship.

In the present study, the prevalence of reflux symptoms was 8.2%. This finding is comparable to that of previous studies that showed a change in the prevalence of GERD in Eastern Asia from 5.2% to 8.5% between 2005 and 2010.23,39 The percentage of subjects with EE among those with GERD was approximately 11% (176 of 1574) in this study, which is lower than that in previous studies (50%).40,41 However, a nationwide multicenter prospective study that included subjects who underwent a health check-up across 40 health-care centers in Korea reported that only 14% of subjects with GERD showed endoscopic evidence of EE.23 This finding could be explained by the hypothesis that EE may have healed in a few patients owing to the widespread use of PPIs, and the percentage of patients with ERD may have been reduced because these patients have been reclassified into the NERD group.42 Notably, the subjects included in our study belonged to a high socioeconomic group, voluntarily participated in medical check-ups, and were more interested in and concerned about their health status, and also had better access to medical care than the general population. Thus, the study subjects may have been more frequently exposed to various drugs including PPIs. The lower percentage of patients belonging to the AEE subtype in this study than in previous studies could be attributed to this fact.

The strengths of this study include the following: (1) The large sample size ensured the robustness of our results. (2) The physician filled the questionnaire administered to all subjects for the assessment of reflux symptoms, which ensured the objectivity and completeness of the symptom evaluation. (3) The analyzed data included findings of endoscopic examinations; thus, we could evaluate the associations of the psychological factors separately and accurately in each subtype of GERD. (4) The STAI was able to assess both the acute and chronic anxiety levels including 2 types of anxiety (trait or chronic anxiety that reflects a person’s permanent characteristics and state or acute anxiety that reflects a recent state).43

The limitations of this study are as follows: (1) As this was a cross-sectional study, we could not establish causal relationships between psychological factors and reflux symptoms. Further prospective longitudinal studies are warranted to elucidate the pattern of causality. (2) This study involved healthy persons who underwent health checkups and did not include the use of PPI for reflux symptoms. Thus, the characteristics of subjects included in this study may differ from those of patients visiting outpatient clinics. (3) This study lacks data about treatment with current anxiolytics or antidepressants. It has been shown that both tricyclic antidepressants, which reduce lower esophageal sphincter pressure, and selective serotonin reuptake inhibitors, which impair esophageal motility, can cause reflux episodes. Benzodiazepines, which are widely used as anti-anxiety medications, can reduce the pain threshold and affect the reflux perception.32 Therefore, it is difficult to definitively conclude whether the presence of reflux symptoms is related to anxiety and depressive symptoms themselves or to the use of specific psychiatric medications. (4) We did not evaluate the interobserver variability in the endoscopic diagnosis of EE, atrophic gastritis, and intestinal metaplasia; however, the 17 gastroenterologists who performed the endoscopic examinations in this study were all board-certified experts with a minimum experience of 10 000 upper endoscopies.

In conclusion, this large cross-sectional study demonstrated that levels of anxiety and depression were higher in subjects with GERD than in controls. Notably, subjects with NERD showed higher levels of anxiety and depression. A multidisciplinary approach to assess and manage these psychological factors is needed for the treatment of GERD.