Literature DB >> 3034351

Multiwell cap assay: a simple objective method for the assessment of leukocyte locomotion in vitro.

A M Evans, J D Chambers, J R Hobbs.   

Abstract

A simple method for evaluating leukocyte locomotion in vitro has been developed and validated for several chemoattractants. The multiwell cap assay (MWCA) comprises chambers constructed from readily available disposable plastics and is quickly assembled, permitting large experimental protocols. Leukocytes which have migrated through a micropore filter are recovered and counted electronically yielding a precise, objective result. Coefficients of variation are approximately 6%.

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Year:  1986        PMID: 3034351     DOI: 10.1007/BF01141025

Source DB:  PubMed          Journal:  Biosci Rep        ISSN: 0144-8463            Impact factor:   3.840


  1 in total

1.  Comparison of LTB4- and C5a-stimulated chemotaxis of isolated human neutrophils: difference revealed by cell migration in thick filters using the multiwell cap procedure.

Authors:  S Psychoyos; S Uziel-Fusi
Journal:  Agents Actions       Date:  1989-06
  1 in total

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