Comparison of indirect immunofluorescence and radioimmunoassay for detecting antibodies against hepatitis A virus of the IgG and IgM classes.

Indirect Immunofluorescence test (IIF) and Radioimmunoassay (RIA) were compared for determining anti-Hepatitis A virus (HAV) IgG and IgM. 142 sera were tested for anti-HAV IgG and 16 for anti-HAV IgM, both with IIF and RIA techniques. The correlation between the results reached 98.6% for anti-HAV IgG and 93.75% for anti-HAV IgM detection, confirming the specificity and sensibility of IIF.