Basma Emad Aboulhoda1, Sherif S Hassan2. 1. Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Egypt. Electronic address: basma.emad@kasralainy.edu.eg. 2. Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Egypt; Department of Medical Education, California University of Sciences and Medicine, School of medicine, San Bernardino, 92408 CA, USA.
Abstract
BACKGROUND AND AIM: The adverse neurological effects of tramadol have recently raised attention. The literature pertaining to studying postnatal cerebellar changes induced by prenatal tramadol is very scanty, thus the current study has been designed to improve understanding of the cerebellar oxidative stress-related alterations associated with tramadol administration during pregnancy in this critical period of neuronal differentiation and synaptic development, thereby highlighting the importance of controlling prenatal prescription of opioids and optimizing care for opioid-dependent pregnant women and their infants. MATERIAL AND METHODS: Twenty pregnant female rats of Sprague Dawley strains were used in the study. Their offspring were divided into two groups: group I (control group) offspring of mothers given saline; group II offspring of mothers given tramadol from the 10th day (D10) of gestation till D21. The pups were sacrificed on the 7th, 14th and 21st postnatal days. Cerebellar specimens were processed for histomorphometric, immunohistochemical and electron microscopic assessment and were evaluated for various oxidative stress parameters. RESULTS: Tramadol administration during pregnancy caused profound structural abnormalities on the post-natal cerebellar cortex and was associated with oxidative stress evidenced by elevation of lipid peroxidation products and inhibition of antioxidant enzyme activities.
BACKGROUND AND AIM: The adverse neurological effects of tramadol have recently raised attention. The literature pertaining to studying postnatal cerebellar changes induced by prenatal tramadol is very scanty, thus the current study has been designed to improve understanding of the cerebellar oxidative stress-related alterations associated with tramadol administration during pregnancy in this critical period of neuronal differentiation and synaptic development, thereby highlighting the importance of controlling prenatal prescription of opioids and optimizing care for opioid-dependent pregnant women and their infants. MATERIAL AND METHODS: Twenty pregnant female rats of Sprague Dawley strains were used in the study. Their offspring were divided into two groups: group I (control group) offspring of mothers given saline; group II offspring of mothers given tramadol from the 10th day (D10) of gestation till D21. The pups were sacrificed on the 7th, 14th and 21st postnatal days. Cerebellar specimens were processed for histomorphometric, immunohistochemical and electron microscopic assessment and were evaluated for various oxidative stress parameters. RESULTS:Tramadol administration during pregnancy caused profound structural abnormalities on the post-natal cerebellar cortex and was associated with oxidative stress evidenced by elevation of lipid peroxidation products and inhibition of antioxidant enzyme activities.
Authors: Madeleine C Allen; Nora K Moog; Claudia Buss; Elizabeth Yen; Hanna C Gustafsson; Elinor L Sullivan; Alice M Graham Journal: Neurotoxicol Teratol Date: 2021-09-30 Impact factor: 4.071
Authors: Giulia Spoto; Greta Amore; Luigi Vetri; Giuseppe Quatrosi; Anna Cafeo; Eloisa Gitto; Antonio Gennaro Nicotera; Gabriella Di Rosa Journal: Front Syst Neurosci Date: 2021-06-10